Selective phosphodiesterase 4 inhibitor roflumilast reduces inflammation and lung injury in models of betacoronavirus infection in mice.

Objective: We aimed to understand the potential therapeutic and anti-inflammatory effects of the phosphodiesterase-4 (PDE4) inhibitor roflumilast in models of pulmonary infection caused by betacoronaviruses.

Methods: Mice were infected intranasally with murine hepatitis virus (MHV-3) or severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Roflumilast was given to MHV-3-infected mice therapeutically at doses of 1 mg/kg or 10 mg/kg, or prophylactically at 10 mg/kg.

The effect of midazolam co-induction on cardiorespiratory variables, myoclonus and etomidate dose requirements in healthy cats.

Objective: To evaluate dose requirements of etomidate for endotracheal intubation, with or without midazolam co-induction, and to describe induction quality and associated cardiorespiratory variables in healthy cats.

Study Design: Randomized prospective experimental study.

Animals: A group of 24 adult neutered cats (17 females, seven males).

Production of nondairy fermented products with chestnut puree as substrate and milk kefir grains or two lactic acid bacteria.

This work aimed to study the production, for the first time, of three fermented products of chestnut puree (CP) with milk kefir grains, a higher nisin-producing (Lactococcus (L.) lactis CECT 539) and a higher lactic acid-producing (Lactobacillus (Lb.) casei CECT 4043) lactic acid bacteria (LAB).

Quality and Regulatory Requirements for the Manufacture of Master Cell Banks of Clinical Grade iPSCs: The EU and USA Perspectives.

The discovery of induced pluripotent stem cells (iPSCs) and protocols for their differentiation into various cell types have revolutionized the field of tissue engineering and regenerative medicine. Developing manufacturing guidelines for safe and GMP-compliant final products has become essential. Allogeneic iPSCs-derived cell therapies are now the preferred manufacturing alternative.