Publications by authors named "F J Benning"
Article Synopsis
- Genetic vascular disorders, particularly multisystemic smooth muscle dysfunction syndrome (MSMDS), result from mutations in the alpha actin isotype 2 gene and can lead to severe health issues such as stroke and early childhood death.
- The research focused on correcting the common R179H mutation using a specially engineered CRISPR-Cas9 enzyme designed for high accuracy, decreasing unintended edits during the gene correction process.
- By utilizing a murine model that mimics human MSMDS symptoms, the study demonstrated that delivering the customized editing tool significantly improved survival and health outcomes in affected mice, indicating potential for lasting treatments in humans.
Genome editing technologies based on DNA-dependent polymerases (DDPs) could offer several benefits compared with other types of editors to install diverse edits. Here, we develop click editing, a genome writing platform that couples the advantageous properties of DDPs with RNA-programmable nickases to permit the installation of a range of edits, including substitutions, insertions and deletions. Click editors (CEs) leverage the 'click'-like bioconjugation ability of HUH endonucleases with single-stranded DNA substrates to covalently tether 'click DNA' (clkDNA) templates encoding user-specifiable edits at targeted genomic loci.
View Article and Find Full Text PDFIn eukaryotes, the essential process of cellular respiration takes place in the cristae of mitochondria. The protein Mic60 is known to stabilize crista junctions; however, how the C-terminal Mitofilin domain of Mic60 mediates cristae-supported respiration remains elusive. Here, we used ancestral sequence reconstruction to generate Mitofilin ancestors up to and including the last opisthokont common ancestor (LOCA).
View Article and Find Full Text PDFObjective: To describe implementation strategies for preventive health measures in SMEs and the effectiveness of the strategies on implementation outcomes.
Methods: A literature search was performed in multiple electronic databases. Studies published between 2000 and 2021 that evaluated the implementation of preventive health measures in SMEs were included.
Baculoviruses are insect-infecting pathogens with wide applications as biological pesticides, in vitro protein production vehicles and gene therapy tools. Its cylindrical nucleocapsid, which encapsulates and protects the circular double-stranded viral DNA encoding proteins for viral replication and entry, is formed by the highly conserved major capsid protein VP39. The mechanism for VP39 assembly remains unknown.
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