Ψ-Footprinting approach for the identification of protein synthesis inhibitor producers.

Today, one of the biggest challenges in antibiotic research is a targeted prioritization of natural compound producer strains and an efficient dereplication process to avoid undesired rediscovery of already known substances. Thereby, genome sequence-driven mining strategies are often superior to wet-lab experiments because they are generally faster and less resource-intensive. In the current study, we report on the development of a novel screening approach to evaluate the genetic potential of bacterial strains to produce protein synthesis inhibitors (PSI), which was termed the protein synthesis inhibitor ('psi') target gene footprinting approach = Ψ-footprinting.

Biological and Physical Clogging in Infiltration Wells: Effects of Well Diameter and Gravel Pack.

Gravity-driven infiltration into the shallow subsurface via small-diameter wells (SDWs), i.e., wells with an inner diameter smaller than 7.

Bioreporters for direct mode of action-informed screening of antibiotic producer strains.

A big challenge in natural product research of today is rapid dereplication of already known substances, to free capacities for the exploration of new agents. Prompt information on bioactivities and mode of action (MOA) speeds up the lead discovery process and is required for rational compound optimization. Here, we present a bioreporter approach as a versatile strategy for combined bioactivity- and MOA-informed primary screening for antimicrobials.

Investigation of the Autoregulator-Receptor System in the Pristinamycin Producer .

Pristinamycin biosynthesis in is governed by a complex hierarchical signaling cascade involving seven different transcriptional regulators (SpbR, PapR1, PapR2, PapR3, PapR4, PapR5, and PapR6). The signaling cascade is triggered by γ-butyrolactone (GBL)-like effector molecules, whereby the chemical structure of the effector, as well as its biosynthetic origin is unknown so far. Three of the pristinamycin transcriptional regulators (SpbR, PapR3, and PapR5) belong to the type of γ-butyrolactone receptor (GBLR).

Influence of recharge rates on steady-state plume lengths.

A large number of potentially contaminated sites reported worldwide require cost- and time-effective assessment of the extent of contamination and the threats posed to the water resources. A significant risk assessment metric for these sites can be the determination of the maximum (i.e.