Publications by authors named "F H Waqas"
Objectives: The prevalence of antimicrobial resistance (AMR) among Gram-negative bacteria is a major global health concern. Resistance to last-resort antibiotics like colistin is particularly alarming. This study reviews how environmental factors have contributed to colistin resistance in the African context, where reports of colistin-resistant Gram-negative organisms are emerging.
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- Early infection dynamics are critical for understanding host-pathogen interactions, but studying these in human lungs is challenging; thus, researchers analyzed human lung tissue cultured from patients with emphysema to observe the immediate responses to various pathogens.
- Pseudomonas aeruginosa was found to induce the most significant changes in RNA expression, particularly affecting microRNA, with influenza A virus (IAV) showing a distinct RNA signature linked to its infection response.
- Both bacterial pathogens elicited similar mRNA expression changes, yet P. aeruginosa's impact was stronger; the study identified six key mRNAs that form the core response to IAV, highlighting differences in how various pathogens trigger lung tissue reactions.
Exosomes carry large cargo of proteins, lipids, and nucleic acids, serving as versatile biomarkers for disease diagnosis and vehicles for drug delivery. However, up to date, no well recognized standard procedures for exosome storage were available for clinical application. This study aimed to determine the optimal storage conditions and the anticoagulants for plasma-derived exosome isolation.
View Article and Find Full Text PDFBackground: Viral and autoimmune encephalitis may present with similar symptoms, but require different treatments. Thus, there is a need for biomarkers to improve diagnosis and understanding of pathogenesis. We hypothesized that virus-host cell interactions lead to different changes in central nervous system (CNS) metabolism than autoimmune processes and searched for metabolite biomarkers in cerebrospinal fluid (CSF) to distinguish between the two conditions.
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