Characterization of amino acid transport in the dairy strain Leuconostoc mesenteroides subsp. mesenteroides CNRZ 1273.

Aims: To identify and characterize amino acid transport in Leuconostoc mesenteroides.

Methods And Results: The transport of labelled amino acids was measured in whole cells of Leuc. mesenteroides CNRZ 1273.

Valine transport and biodiversity of Leuconostoc wild strains from French raw milk cheeses.

The rate of L-valine transport in whole cells of Leuconostoc was at the maximum at 30 degrees C, pH 6.0 in the presence of an energy source. Transport was inhibited by 40-55%, in the presence of the ionophores (valinomycin, nigericin or monensin), and uncouplers (carbonyl cyanide-m-chloro-phenylhydrazone or 2,4-dinitrophenol) confirming the previously described delta p-driven branched-chain amino acid transport system described in cytoplasmic membranes (Winters et al.