Publications by authors named "F Egberts"
Article Synopsis
- Consumption of raw or undercooked meat can lead to infections from Toxoplasma gondii, but salt and additives in meat may impact the parasite's survival.
- A new in vitro method was developed to replace the mouse bioassay for testing how salting affects T. gondii viability, using meat samples from infected sheep to evaluate parasite growth in cultured cells.
- The study found that untreated meat samples had viable T. gondii, while higher salt concentrations (2.7% NaCl) significantly reduced parasite growth, demonstrating the effectiveness of the new in vitro method for assessing T. gondii viability.
Background: Whether melanoma in histological contiguity with a naevus [naevus-associated melanoma (NAM)] is distinctly different from melanoma arising de novo remains unclear.
Objectives: To determine whether the characteristics of de novo melanoma differ from NAM and are not due to naevus obliteration in thicker tumours.
Methods: We conducted a multicentre retrospective study of de novo melanoma and NAM in seven referral centres in Europe, Australia and the USA between 2006 and 2015.
Purpose: The purpose of this study was to validate the results of an 11-gene expression profiling (GEP) assay which aims to improve the precision of individual prognosis beyond conventional American Joint Committee on Cancer staging for patients with cutaneous melanoma.
Methods: The reverse transcriptase polymerase chain reaction test of 11 prospectively selected genes was performed on 291 formalin-fixed, paraffin-embedded primary tumours of patients with stage I-III cutaneous melanoma. The expression levels of eight prognostic and three reference genes were used in a predefined algorithm to calculate a numerical score (-0.