Publications by authors named "F Duquesne"
In 2018, a T. asinigenitalis strain (MCE663) was isolated in a Persian onager tested for contagious equine metritis (CEM) in a United Kingdom (UK) zoo. This bacterium had never been reported in the UK and Multilocus Sequence Typing described a new atypically divergent ST (ST60).
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- - The European Union and World Organisation for Animal Health now endorse real-time PCR as an effective method for detecting contagious equine metritis (CEM), alongside traditional culture methods.
- - A network of 20 approved laboratories in France was established in 2017 to conduct CEM detection via real-time PCR, with ongoing proficiency tests initiated to evaluate their performance.
- - From 2017 to 2021, proficiency tests showed that 99.20% of qualitative results were accurate, highlighting that direct lysis DNA extraction yielded more favorable results than other methods, although the differences were not statistically significant.
The cultural diagnosis of the causal agent of contagious equine metritis (Taylorella equigenitalis) using transport swabs is challenging. Swabs must be placed in Amies charcoal medium, refrigerated during transport, and plated out at the laboratory no later than 48 h after sampling. In this study, the viability of T.
View Article and Find Full Text PDFBackground: Three horse mares inadvertently inseminated with semen from a Tayorella asinigenitalis-positive Jack donkey developed severe, purulent endometritis whereas two Jenny donkeys mated naturally to the same Jack donkey did not develop clinical signs of infection.
Objectives: To isolate and identify the causative agent.
Study Design: Case report.
Objectives: Study of the rifampicin resistance of Rhodococcus equi strains isolated from French horses over a 20-year period.
Methods: Rifampicin susceptibility was tested by disk diffusion (DD) and broth macrodilution methods, and rpoB gene sequencing and MLST were performed on 40 R. equi strains, 50.