Cloning and expression of the canine interferon-gamma gene.

The canine interferon-gamma (cIFN-gamma) chromosomal gene was isolated from a recombinant library, containing the entire dog genome, by screening with a rat IFN-gamma genomic probe. The structural organization of the cIFN-gamma gene closely resembles that of the human, murine, and rat IFN-gamma genes. It contains three intervening sequences and encodes a signal sequence of 23 amino acids followed by a mature protein of 143 amino acids.

Simian virus 40 mutants carrying extensive deletions in the 72-base-pair repeat region.

Simian virus 40 mutants were constructed with deletions at the late side of the origin of DNA replication by partial Bal 31 digestion at the SphI site or at the PvuII site. Some of these mutants lost virtually all of both 72-base-pair repeat segments ("enhancer" sequences) and exhibited a decrease in viability from 20-to 300-fold; one particular mutant, dl1852, even showed a reduction of almost 10(4)-fold. The very poorly growing deletion mutants were unstable and gave rise to DNA rearrangements upon further growth.

Deletion mapping of the inducible promoter of human IFN-beta gene.

Human fibroblast cells produce beta-type interferon only in response to viral infection or treatment with an inducer such as poly(rI) X poly(rC); this event is most probably controlled at the transcriptional level (for review see ref. 1). To study the induction process, we inserted the human fibroblast interferon (IFN-beta) gene, with or without its promoter region, into recombinant simian virus 40 (SV40) plasmid vectors which subsequently were transfected into monkey AP-8 cells.

Cloning and structure of the human interleukin 2 chromosomal gene.

Southern hybridization using 32P-labelled human interleukin 2 (IL2) cDNA probes revealed the existence of a single human IL2 gene. Five clones containing the human IL2 chromosomal gene were isolated from two different human DNA libraries cloned in either lambda Charon 4A or L47 phages. Analysis of the clones showed that they contained different, overlapping portions of human DNA which were derived from the same chromosomal segment.

Complete nucleotide sequence of bacteriophage MS2 RNA: primary and secondary structure of the replicase gene.

Bacteriophage MS2 RNA is 3,569 nucleotides long. The nucleotide sequence has been established for the third and last gene, which codes for the replicase protein. A secondary structure model has also been proposed.