Publications by authors named "F Delvigne"
Article Synopsis
- * Significant fluctuations in the consumption of these sugars were observed as early as the 50th generation, which were not solely due to the emergence of low-consumption clones but instead linked to variations in the number of gene copies responsible for sugar assimilation.
- * The findings suggest that while the yeast shows some genetic and metabolic instability, these issues could potentially worsen in more extreme industrial conditions, negatively impacting production efficiency.
Cell-to-cell heterogeneity presents challenges across various fields, from biomedicine to bioproduction, where precise cellular responses are vital. While single cell technologies have significantly enhanced our understanding of population heterogeneity, the predominant focus has been on monitoring intracellular compounds. Recognizing the added complexity introduced by the secretion system, in this review, we first provide a systematic overview of the distinct steps necessary for driving protein secretion.
View Article and Find Full Text PDFDecoupling cell formation from recombinant protein synthesis is a potent strategy to intensify bioprocesses. Escherichia coli strains with mutations in the glucose uptake components lack catabolite repression, display low growth rate, no overflow metabolism, and high recombinant protein yields. Fast growth rates were promoted by the simultaneous consumption of glucose and glycerol, and this was followed by a phase of slow growth, when only glucose remained in the medium.
View Article and Find Full Text PDFArticle Synopsis
- The main idea is to control bioprocesses by giving cells exactly what they need, when they need it, instead of just watching the environmental conditions.
- Scientists used special biosensors and online technology to check how bacteria feel and what they need to grow better.
- They tested this system with E. coli and found that it improved the way bacteria are grown, making the process faster and more effective.
Article Synopsis
- Isogenic cell populations can adapt to stress by switching to different phenotypes, but this variability is problematic for applications like bioproduction and synthetic biology.
- The study investigates how various systems (like bacteria and yeast) diversify under stress, revealing that the fitness cost of switching phenotypes correlates with population dynamics.
- A stochastic model identifies three diversification patterns—constrained, dispersed, and bursty—based on switching costs, and a tool called Segregostat allows for better control over these patterns for more predictable cellular behavior.