Development of a triplex crystal digital RT-PCR for the detection of PHEV, PRV, and CSFV.

Porcine hemagglutinating encephalomyelitis virus (PHEV), porcine pseudorabies virus (PRV), and classical swine fever virus (CSFV) are currently prevalent worldwide and cause similar neurological symptoms in infected pigs. It is very important to establish a detection method that can rapidly and accurately detect and differentiate these three viruses. Targeting the PHEV N gene, PRV gB gene, and CSFV 5' untranslated region (5'UTR), three pairs of specific primers and probes were designed, and a triplex crystal digital reverse transcription-PCR (cdRT-PCR) was developed to detect PHEV, PRV, and CSFV.

Robust pattern retrieval in an optical Hopfield neural network.

Hopfield neural networks (HNNs) promise broad applications in areas such as combinatorial optimization, memory storage, and pattern recognition. Among various implementations, optical HNNs are particularly interesting because they can take advantage of fast optical matrix-vector multiplications. Yet their studies so far have mostly been on the theoretical side, and the effects of optical imperfections and robustness against memory errors remain to be quantified.

The characteristics of phthalate acid esters and bisphenol A in PM of a petrochemical city: Concentrations, compositions, and health risk assessment in Dongying.

Phthalate acid esters (PAEs) and bisphenol A (BPA) are recognized as common endocrine disruptors associated with various adverse effects on human health. However, limitations in existing systematic studies, particularly in air detection, have raised concerns about potential health risks from inhalation exposure. In this study, PM samples were collected in Dongying, a petrochemical city, from October 27 to December 6, 2021.

A Quadruplex RT-qPCR for the Detection of African Swine Fever Virus, Classical Swine Fever Virus, Porcine Reproductive and Respiratory Syndrome Virus, and Porcine Pseudorabies Virus.

African swine fever virus (ASFV), classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), and porcine pseudorabies virus (PRV) induce similar clinical signs in infected pigs, including hyperthermia, anorexia, hemorrhage, respiratory distress, neurological symptoms, and/or abortions in pregnant sows. The differential diagnosis of these diseases relies on laboratory examinations. In this study, a quadruplex RT-qPCR was established using four pairs of specific primers and probes aimed at the (p72) gene of ASFV, the 5' untranslated region () of CSFV, the gene of PRRSV, and the gene of PRV for the detection and differentiation of ASFV, CSFV, PRRSV, and PRV.

A facile optical fiber-embedded microfluidic biochip for rapid and sensitive detection of microRNA-let-7a in serum.

A novel hybridization chain reaction (HCR) powered optical fiber-embedded microfluidic biochip (HCR-FMB) has been constructed for ultrafast and sensitive detection of lethal-7a (let-7a) in serum. By integrating HCR, fluorescence energy resonant transfer, and evanescent wave fluorescence principle, the HCR-FMB enables detecting let-7a with satisfactory limit of detection of 100.0 pM within 6 min at room temperature, and demonstrates excellent specificity.