Ethylene, a Signaling Compound Involved in Seed Germination and Dormancy.

The present review is focused on current findings on the involvement of ethylene in seed biology. The responsiveness of seeds to ethylene depends on the species and the dormancy status, improving concentrations ranging from 0.1 to 200 μL L.

ERFVII action and modulation through oxygen-sensing in Arabidopsis thaliana.

Oxygen is a key signalling component of plant biology, and whilst an oxygen-sensing mechanism was previously described in Arabidopsis thaliana, key features of the associated PLANT CYSTEINE OXIDASE (PCO) N-degron pathway and Group VII ETHYLENE RESPONSE FACTOR (ERFVII) transcription factor substrates remain untested or unknown. We demonstrate that ERFVIIs show non-autonomous activation of root hypoxia tolerance and are essential for root development and survival under oxygen limiting conditions in soil. We determine the combined effects of ERFVIIs in controlling gene expression and define genetic and environmental components required for proteasome-dependent oxygen-regulated stability of ERFVIIs through the PCO N-degron pathway.

Label-Free Quantitative Proteomics Reveal the Involvement of PRT6 in Seed Responsiveness to Ethylene.

In , the breaking of seed dormancy in wild type (Col-0) by ethylene at 100 μL L required at least 30 h application. A mutant of the proteolytic N-degron pathway, lacking the E3 ligase (), was investigated for its role in ethylene-triggered changes in proteomes during seed germination. Label-free quantitative proteomics was carried out on dormant wild type Col-0 and seeds treated with (+) or without (-) ethylene.

In-Depth Proteomic Analysis of the Secondary Dormancy Induction by Hypoxia or High Temperature in Barley Grains.

In barley, incubation of primary dormant (D1) grains on water under conditions that do not allow germination, i.e. 30°C in air and 15°C or 30°C in 5% O2, induces a secondary dormancy (D2) expressed as a loss of the ability to germinate at 15°C in air.