Publications by authors named "F Carvalheiro"
Lytic polysaccharide monooxygenases (LPMOs) catalyze oxidative cleavage of crystalline polysaccharides such as cellulose and are crucial for the conversion of plant biomass in Nature and in industrial applications. Sunlight promotes microbial conversion of plant litter; this effect has been attributed to photochemical degradation of lignin, a major redox-active component of secondary plant cell walls that limits enzyme access to the cell wall carbohydrates. Here, we show that exposing lignin to visible light facilitates cellulose solubilization by promoting formation of HO that fuels LPMO catalysis.
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- Exhausted olive pomace (EOP) is a significant agro-industrial waste that contains valuable phenolic compounds and mannitol, making its bioactive extraction a key step for its valorization.
- A study utilized ultrasound-assisted extraction (UAE) to optimize conditions for extracting these compounds, determining the best results with 40% acetone, 8.6% solids, and 43 minutes of extraction time.
- Additionally, a probe-type UAE method was tested to reduce extraction time, successfully achieving similar phenolic compound levels in just 12 minutes, which may lower costs in industrial applications.
The yeast NCYC 921 was used for lipid production, using biomass hydrolysate as carbon source. The hydrolysate was obtained by enzymatic hydrolysis of biomass (at high solids loading) previously subjected to a hydrothermal pre-treatment. Afterwards was grown on sp.
View Article and Find Full Text PDFBanana's pseudostem pulp (BPP) is a potential by-product obtained in the mechanical fiber extraction of banana's pseudostem. Its chemical characterization revealed to have an interesting composition, with a high polysaccharides content and low content in lignin, which makes it particularly relevant for the biorefinery's biochemical platform. Autohydrolysis pretreatment, studied under isothermal (140 °C) and non-isothermal conditions (140-220 °C), yielded oligosaccharides, mainly gluco-oligosaccharides, as the main soluble products.
View Article and Find Full Text PDFThis work aims to evaluate the prebiotic potential of oligosaccharides (OS) obtained from autohydrolysis of olive tree pruning biomass (OTPB). Two selected fractions (F1 and F2) were characterized and used in in vitro fermentations by two Bifidobacterium spp. (B.
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