Multiplex digital PCR for the simultaneous quantification of a miRNA panel.

Background: microRNAs (miRNAs) are small non-coding RNAs regulating gene expression. They have attracted significant interest as biomarkers for early diagnosis, prediction and monitoring of treatment response in many diseases. As individual miRNAs often lack the required sensitivity and specificity, miRNA signatures are developed for clinical applications.

A comparison of veterinary classification schemes, serum ascites albumin gradient, and Boyer's criteria in discriminating transudates from exudates in ascitic dogs.

Objectives: To assess the parameters utilised by and the diagnostic performance of two traditional veterinary classification schemes (named A and B) based on ascites total protein and total nucleated cell count, the Boyer's criteria based on ascites lactic dehydrogenase activity, its serum ratio and the serum total protein, a simplified Boyer's criteria based on ascites lactic dehydrogenase activity and serum total protein only, and finally the serum-ascites albumin gradient in discriminate the pathophysiological origin of the ascites formation in dogs.

Materials And Methods: Cross-sectional study including 291 client-owned dogs with ascites. Ascites aetiology was used to classify the pathophysiology of its formation.

Intraoperative surgical navigation as a precision medicine tool in sinonasal and craniofacial oncologic surgery.

Introduction: Recent evidence supports the efficacy of surgical navigation (SN) in improving outcomes of sinonasal and craniofacial oncologic surgery. This study aims to demonstrate the utility of SN as a tool for integrating surgical, radiologic, and pathologic information. Additionally, a system for recording and mapping biopsy samples has been devised to facilitate sharing of spatial information.

Retrospective evaluation of the short-term response of human intravenous immunoglobulin therapy in the management of canine immune-mediated thrombocytopenia (2010-2015): 27 cases.

Objective: To describe the short-term response, early prognostic markers, and survival after treatment of canine immune-mediated thrombocytopenia (ITP) with human intravenous immunoglobulin (hIVIG) and methylprednisolone.

Design: Retrospective cohort study.

Settings: Private referral veterinary medical center.