Human mixed infections of Leishmania spp. and Leishmania-Trypanosoma cruzi in a sub Andean Bolivian area: identification by polymerase chain reaction/hybridization and isoenzyme.

Parasites belonging to Leishmania braziliensis, Leishmania donovani, Leishmania mexicana complexes and Trypanosoma cruzi (clones 20 and 39) were searched in blood, lesions and strains collected from 28 patients with active cutaneous leishmaniasis and one patient with visceral leishmaniasis. PCR-hybridization with specific probes of Leishmania complexes (L. braziliensis, L.

Serologic and parasitologic demonstration of Trypanosoma cruzi infections in an urban area of central Mexico: correlation with electrocardiographic alterations.

Trypanosoma cruzi infection in central Mexico has not been fully documented, yet some data suggest its presence. In this work, sera from 211 subjects living in the state of Morelos and at risk of T. cruzi infection due to their living in contact with the vector were analyzed for the presence of antibodies to a total antigen extract of a Mexican isolate of T.

Ecogenetics of Triatoma sordida and Triatoma guasayana (Hemiptera: reduviidae) in the Bolivian chaco.

Triatoma guasayana and two putative cryptic species pertaining to T. sordida complex (named groups 1 and 2) occur in sympatry in the Bolivian Chaco. Using multilocus enzyme electrophoresis and subsequent genetic analysis, our work assesses their population distribution and dispersal capacity in domestic, peridomestic, and silvatic environments.

Cryptic speciation in Triatoma sordida (Hemiptera:Reduviidae) from the Bolivian Chaco.

Eight natural Bolivian populations of two closely related species of Triatominae, Triatoma sordida and T. guasayana, were analysed by multilocus enzyme electrophoresis at 20 loci. Both species were readily separated and no natural hybrid was observed.

Low probability of transmission of Trypanosoma cruzi to humans by domiciliary Triatoma sordida in Bolivia.

The role of Triatoma sordida in the domestic transmission of Trypanosoma cruzi was assessed in 7 rural localities in Velasco Province, Department of Santa Cruz, Bolivia. Tri. sordida, the only triatomine species identified in these localities, was found inside 58.