Biochemical characteristics of collagen produced by long term cultivated amniotic fluid cells.

The synthesis of the most common type I of collagen was proven in human amniotic fluid cell culture with fibroblast morphology in the cells as well as in the culture medium. Mesenchymal origin of long term cultivated amniotic fluid cells is indicated and the possibility of prenatal investigation of hereditary disorders of connective tissue is pointed out.

Biosynthesis of collagen during the life cycle of human diploid cell lines.

In the course of the active phase of growth collagen synthesis appears relatively stable and decreases when the culture passes into the degenerative phase III. The increase of cell nitrogen indicates the increasing accumulation of cell proteins with their aging in vitro. The ratio of soluble to insoluble collagen is relatively constant in fibroblast cultures from different tissues, periods of development during the active phase of growth.