Proteomic diversity in bacteria: Insights and implications for bacterial identification.

Mass spectrometry-based proteomics has revolutionized bacterial identification and elucidated many molecular mechanisms underlying bacterial growth, community formation, and drug resistance. However, most research has been focused on a few model bacteria, overlooking bacterial diversity. In this study, we present the most extensive bacterial proteomic resource to date, covering 303 species, 119 genera, and five phyla with over 636,000 unique expressed proteins, confirming the existence of over 38,700 hypothetical proteins.

Gemcitabine and ATR inhibitors synergize to kill PDAC cells by blocking DNA damage response.

The DNA-damaging agent Gemcitabine (GEM) is a first-line treatment for pancreatic cancer, but chemoresistance is frequently observed. Several clinical trials investigate the efficacy of GEM in combination with targeted drugs, including kinase inhibitors, but the experimental evidence for such rationale is often unclear. Here, we phenotypically screened 13 human pancreatic adenocarcinoma (PDAC) cell lines against GEM in combination with 146 clinical inhibitors and observed strong synergy for the ATR kinase inhibitor Elimusertib in most cell lines.

PTMNavigator: interactive visualization of differentially regulated post-translational modifications in cellular signaling pathways.

Post-translational modifications (PTMs) play pivotal roles in regulating cellular signaling, fine-tuning protein function, and orchestrating complex biological processes. Despite their importance, the lack of comprehensive tools for studying PTMs from a pathway-centric perspective has limited our ability to understand how PTMs modulate cellular pathways on a molecular level. Here, we present PTMNavigator, a tool integrated into the ProteomicsDB platform that offers an interactive interface for researchers to overlay experimental PTM data with pathway diagrams.

A convergent mixed methods to study registration on kidney transplantation waiting list refusal by women and men on dialysis in France.

Not all patients on dialysis want to be registered on the kidney transplantation (KT) waiting list and undergo transplantation. The aim of this convergent mixed methods study was to determine the features of patients refusing to be registered on the KT waiting list and the reasons. Quantitative data on all 2017-2019 incident 18-85-year-old dialysis patients, eligible for KT, were extracted from the REIN registry in France.

High-fidelity color characterization in virtual reality across head mounted displays, game engines, and materials.

We present a comprehensive colorimetric analysis of three head mounted displays (HMDs) - HTC Vive Pro Eye, Pimax 8K X DMAS, and Varjo Aero - focusing on their color calibration and uniformity across different game engines (Unity and Unreal) and for different materials/shaders. We developed a robust methodology combining hardware and software tools, including spectroradiometry and imaging colorimetry, to characterize and calibrate these HMDs for accurate color reproduction. The study showcases substantial advancements in colorimetric accuracy, with a reduction in the average deltaE00 of 90 or more across all tested HMDs and conditions.