The nationwide 'ZNIEFF' inventory in France: an open dataset of more than one million species data in zones of high ecological value.

Background: In France, a 'natural zone of ecological, faunistic or floristic value' (Zone Naturelle d'Intérêt Écologique, Faunistique et Floristique - ZNIEFF) is a natural area, regionally known for its remarkable ecological characteristics. The ZNIEFF inventory is a naturalist and scientific survey programme launched in 1982 by the Ecology Ministry, with support from the French National Museum of Natural History (MNHN).

New Information: This paper describes the ZNIEFF national dataset, which comprises 1,013,725 data for various animal (38%), plant (59%) and fungal (2%) species in terrestrial and marine zones (May 2020).

Pyrene sulphonyl chloride as a reagent for quantitation of oestrogens in human serum using HPLC with conventional and laser-induced fluorescence detection.

A highly sensitive HPLC method with conventional and laser-induced fluorescence detection for the analyses of oestrogens is described. beta-oestradiol (beta-ES) was chosen as a model and derivatized with pyrenesulphonyl chloride (PSCL), a novel derivatizing reagent, using a two-phase system with tetrapentylammonium bromide (TPABR) as a phase transfer catalyst. Derivatization was complete in 30 s at room temperature and concentrations as low as 5 x 10(-10) M could be derivatized and detected.

A comparison of fluorescence versus chemiluminescence detection for analysis of the fluorescamine derivative of histamine by HPLC.

Fluorescence and chemiluminescence detection were compared for HPLC analysis of the fluorescamine derivative of histamine. The kinetic behaviour of the chemiluminescent response for the derivative was characterized in a static system. An HPLC method was optimized for the derivative using fluorescence detection.

Stability of phenobarbital N-glucosides: identification of hydrolysis products and kinetics of decomposition.

The two diastereomers of 1-(1-beta-D-glucopyranosyl)phenobarbital, (1A) and (1B), decompose to 1-(1-beta-D-glucopyranosyl)-3-(2-ethyl-2-phenylmalonyl)urea (2A or 2B) followed by decarboxylation to 1-(1-beta-D-glucopyranosyl)-3-(2-phenylbutyryl)urea (3A and 3B) under physiological conditions of temperature and pH. The sigmoidal pH-rate profile and the Arrhenius parameters indicate that degradation takes place by hydroxide ion attack on the undissociated and monoanion forms of 1A and 1B. The rates of hydrolysis of the nonionized species of 1A and 1B are more than two orders of magnitude faster than those of common 5,5-disubstituted or 1,5,5-trisubstituted barbiturates.