An animal model for the study of arterial thrombosis.

1. Thrombus formation induced by electrical stimulation of the carotid artery was investigated in anesthetized rabbits and rats. Occlusive Grade III thrombi were produced consistently in 34 normal New Zealand rabbits and 58 untreated albino Wistar rats.

[Intestinal absorption of glucose in anesthetized rats. I. Standardization of a method for screening of oral hypoglycemic agents].

This paper presents a method for the screening of natural hypoglycaemic drugs that interfere with the intestinal absorption of glucose. Luminal perfusion of the small intestine (whole length) was carried out on 24 h fasted adult Wistar rats, anaesthetized with sodium pentobarbital. Two rubber Nelaton cannulae were introduced into the organ, the first at the proximal end of the duodenum, just after the pylorus and a second larger one near the ileo-cecal valve.

Do platelets migrate into inflammatory exudates?

Rat platelets and the corresponding rabbit IgG antibodies mixed in polyester sponges implanted under the skin of normal animals caused an enhanced 24-h inflammatory response and a significant increase of prostaglandins in the exudate. These experimental results contrast with those obtained for normal rats implanted with sponges containing only IgG anti-platelet antibodies. The present experiments show that circulating platelets were not concentrated at the inflamed site and can hardly be considered of importance in acute inflammation.

Failure to identify 'thrombocytolysin' (a spasmogenic factor released from platelets immunoreaction) with anaphylatoxin.

Horse platelets release an unidentified smooth muscle contracting substance after lysis by antiserum and complement. Since the active factor (thrombocytolysin) does not produce tachyphylactic response of the guinea-pig ileum it seems that it is not related to anaphylatoxins.