[Coding part of the son gene small transcript contains four areas of complete tandem repeats].

Overlapping cDNA clones for the smaller son gene transcript were isolated by several steps of human placenta cDNA library walking. Here we report the structure of repeats that exist at the long open reading, present in the structure of this smaller (5.6 kb) transcript of human gene son.

[Identification of a protein product of a novel human gene SON and the biological effect upon administering a changed form of this gene into mammalian cells].

We have investigated the functional activity of human son gene, that possesses the homology to mos and myc genes. Specific antibodies (antiserum) were raised to synthetic peptide, that corresponds to son-protein 943-963 amino acid residues. With this antiserum the presence of son-protein was showed in lysates of cultured human cells transformed by adenovirus type 5, RAT 2 cells and primary human embryonic fibroblasts.

[Segments of the human genome, containing analogs of oncogenes and retrovirus genes. 4. Primary structure of the mos-related AO section of the ORAgp5 locus].

AO region (884 b.p.) of ORAgp5 locus has been sequenced and proved to contain mos-related regions, as well as fragments structurally similar to proretroviral elements and Alu repeats.

[Decoding of the primary structure of the son3 region in human genome: identification of a new protein with unusual structure and homology with DNA-binding proteins].

From the human embryonic cDNA library a son3 transcript was cloned and sequenced (1454 base pairs). Determination of its sequence revealed only one open reading frame, whereas five other frames contained a number of termination codons. Translation of the son3 sequence in the unique open reading frame into the amino acid sequence by computer and comparison with the NBRF protein data bank showed that the son3 fragment codes for a new previously unknown polypeptide with the following properties: a) it contains a cluster of short tandemly arranged repeats 7-12 amino acid in length located in the middle part of son3; b) it comprises a region homologous to DNA binding structural proteins (for example, gallin, 55%) and to regulatory proteins coded by the family of proto-oncogene myc; c) it comprises a region homologous to the oncoprotein coded by proto-oncogene mos (human, murine).