Aflatoxin detected in human breast milk by immunoassay.

Several epidemiological studies have shown a positive association between dietary exposure to aflatoxin (AF) and an increased incidence of primary hepatocellular carcinoma (PHC). One area in which little information is available is the exposure of newborn children to AF in human breast milk. We report the development, validation and application of an enzyme-linked immunosorbent assay (ELISA) to the detection of AF in human breast milk.