The biological activity of γ-FeO magnetic nanoparticles (MNPs), obtained by the laser target evaporation technique, was studied, with a focus on their possible use in biosensor applications. The biological effect of the MNPs was investigated in vitro on the primary cultures of human dermal fibroblasts. The effects of the MNPs contained in culture medium or MNPs already uptaken by cells were evaluated for the cases of the fibroblast's proliferation and secretion of cytokines and collagen.
View Article and Find Full Text PDFTwo series of composite polyacrylamide (PAAm) gels with embedded superparamagnetic FeO or diamagnetic AlO nanoparticles were synthesized, aiming to study the direct contribution of the magnetic interactions to the ferrogel biocompatibility. The proliferative activity was estimated for the case of human dermal fibroblast culture grown onto the surfaces of these types of substrates. Spherical non-agglomerated nanoparticles (NPs) of 20-40 nm in diameter were prepared by laser target evaporation (LTE) electrophysical technique.
View Article and Find Full Text PDFThe static magnetic field was shown to affect the proliferation, adhesion and differentiation of various types of cells, making it a helpful tool for regenerative medicine, though the mechanism of its impact on cells is not completely understood. In this work, we have designed and tested a magnetic system consisting of an equidistant set of the similar commercial permanent magnets (6 × 4 assay) in order to get insight on the potential of its experimental usage in the biological studies with cells culturing in a magnetic field. Human dermal fibroblasts, which are widely applied in regenerative medicine, were used for the comparative study of their proliferation rate on tissue culture polystyrene (TCPS) and on the polyacrylamide ferrogels with 0.
View Article and Find Full Text PDFAnodization of titanium implants is accompanied by the formation of titanium oxide nanotubes improving osseointegration. An excessive fibroblast adhesion on the surface might lead to the formation of fibrous capsule resulting in implant rejection. In our research, we demonstrated that the adhesion activity of human dermal fibroblasts on anodized surface was not elevated, which is promising for the use of titanium with nanotube-layered surface for implantology.
View Article and Find Full Text PDFIron oxide γ-Fe₂O₃ magnetic nanoparticles (MNPs) were fabricated by laser target evaporation technique (LTE) and their structure and magnetic properties were studied. Polyacrylamide (PAAm) gels with different cross-linking density of the polymer network and polyacrylamide-based ferrogel with embedded LTE MNPs (0.34 wt.
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