Syneretic response to incremental pressures in calf lenses.

Purpose: Syneretic response to pressure variation of 1 atm or more has been demonstrated previously in bovine and human lenses with nuclear magnetic resonance (NMR) relaxation measurements. This study is designed to prove that a similar response is observable in smaller pressure increments closer to the normal physiological range.

Methods: Excised calf eyes were enucleated, the lenses dissected out, and immersed in medium.

Alterations in human vitreous humour following cataract extraction.

Cataract extraction is associated with the risk of posterior vitreous detachment, macular edema and retinal detachment possibly as a result of a disturbance to the vitreous body during surgery. While it is common for lens cortical fiber debris to leak into the vitreous humour during cataract extraction, the extent to which the vitreous humour is altered post-surgery is unknown. The current study examines the integrity of the vitreous humour of pseudophakic and phakic human donor eyes by comparing the proteome, the viscosity and the size distribution of macromolecules in different regions of the vitreous humour from human pseudophakic and phakic donor eyes.

Light scattering in lens research: an essay on accomplishments and promises.

This paper briefly reviews light scattering methodologies in lens research. In the phenomenological sense cataract formation (lens opacities or turbidities) in its early stages can be described by enhanced scattering of light. In the analytical sense information is obtained on the molecular entities involved in light scattering.

Regional mapping of molecular components of human liquid vitreous by dynamic light scattering.

The distribution of particle sizes was studied in six regions of human liquid vitreous samples. Particle sizes were calculated from dynamic light scattering measurements as hydrodynamic diameter of molecules. In general, particle sizes increased progressively from anterior (near lens) to posterior (near retina).

Syneretic response of aging normal human lens to pressure.

Purpose: The study was designed to observe whether a reversible syneretic response to pressure is operative in normal human lenses and whether such a response demonstrates a uniform age dependence.

Methods: Seven sections (from the anterior outer cortex to the posterior outer cortex) of 10 human lenses were imaged at 2 atmospheres (atm) pressure and the T(1) (spin-lattice) and T(2) (spin-spin) relaxation data on each section were collected. The pressure was then released and NMR relaxographic data were collected under 1 atm.