Publications by authors named "F A Aulicino"
Article Synopsis
- * The study found that using alternative viral promoters, specifically Orf-13 and Orf-81, significantly reduced VSV-G expression, improving the stability and morphology of BVs.
- * With optimized VSV-G levels, BVs maintained efficient gene delivery and CRISPR editing capabilities, demonstrating that less VSV-G expression can actually enhance the overall effectiveness and stability of the vectors. *
Efficient genome editing by using CRISPR technologies requires simultaneous and efficient delivery of multiple genetically encoded components to mammalian cells. Amongst all editing approaches, prime editing (PE) has the unique potential to perform seamless genome rewriting, in the absence of DNA double-strand breaks (DSBs). The cargo capacity required for efficient PE delivery to mammalian cells stands at odd with the limited packaging capacity of traditional viral delivery vectors.
View Article and Find Full Text PDFArticle Synopsis
- CRISPR-Cas technologies have transformed genetic engineering, showing potential in treating genetic diseases like chronic kidney disease (CKD), which can be caused by different mutations.
- Recent advances in genomic sequencing combined with CRISPR enable precise correction of mutations, especially in monogenic diseases, offering hope for treating conditions such as polycystic kidney disease and Alport syndrome.
- Innovations like prime editing and base editing improve genome editing efficiency and specificity without causing harmful DNA breaks, but challenges remain in developing effective delivery methods for therapies.
Triple-negative breast cancer (TNBC) is characterised by its aggressiveness and resistance to chemotherapy, demanding the development of effective strategies against its unique characteristics. Derived from lapacho tree bark, β-lapachone (β-LP) selectively targets cancer cells with elevated levels of the detoxifying enzyme NQO1. Hydroxytyrosol (HT) is a phenolic compound derived from olive trees with important anticancer properties that include the inhibition of cancer stem cells (CSCs) and metastatic features in TNBC, as well as relevant antioxidant activities by mechanisms such as the induction of NQO1.
View Article and Find Full Text PDFCRISPR-based DNA editing technologies enable rapid and accessible genome engineering of eukaryotic cells. However, the delivery of genetically encoded CRISPR components remains challenging and sustained Cas9 expression correlates with higher off-target activities, which can be reduced via Cas9-protein delivery. Here we demonstrate that baculovirus, alongside its DNA cargo, can be used to package and deliver proteins to human cells.
View Article and Find Full Text PDF