[Acute hepatotoxicity with intermediate-dose methotrexate in children with leukemia and non-Hodgkin's lymphoma].

In the period of 1.1.1988 to 1.

[Results and experiences with a modified BFM protocol for treatment of recurrences in children with acute lymphoblastic leukemia in East German areas].

Between 1988 and 1990, 55 patients with first relapses of acute lymphoblastic leukemia (ALL) were treated with a modified BFM-protocol (ALL REZ I/88). The patients were divided according to time and site of relapse: relapses with bone marrow involvement up to 6 months after stopping front line therapy (group A), relapses with bone marrow involvement beyond 6 month after therapy (group B) and isolated extramedullary relapses at any time (group C). During therapy the patients received alternating courses of polychemotherapy including infusions of intermediate dose methotrexate (1 g/m2 in 36 hours).

[Experiences with modified BFM protocols in the treatment of children with acute lymphoblastic leukemia (ALL) in East Germany 1981-1991].

Between September and August 1991 818 previously untreated children and adolescents up to 18 years of age with acute lymphoblastic leukemia were entered into two modified BFM-protocols. Patients with B-ALL were excluded. From 1981 to 1987 524 patients were entered into the randomized multicenter study ALL VII/81 (modified ALL-BFM 81 protocol).

[Therapy of acute myeloid leukemia in children--results of the AML II/87 multicenter study].

Fifty seven patients entered the cooperative study AML II/87 of the working group "Pediatric Hematology and Oncology" of East Germany. Two patients with initial hyperleukocytosis died prior therapy. 13 patients died within the first 4 weeks of therapy, 3 patients did not respond to therapy, and one patient is not yet in remission.

[Rare inflammatory reactions in infants and their correlation with pathomorphologic alterations of the immune system].

Autopsy-findings in infants with rare inflammatory disorders or infectious diseases esp. opportunistic infections may suggest defects of the unspecific and specific defence. The histopathological appearances of different lesions were correlated with possible underlying alterations or generalized defects of the immune system.

[Severe aplastic anemia: indications and limits of biological immunosuppression].

Diagnostic criteria++ and differential therapy of SAA are characterized. A record of ATG/ALG-present modus is demonstrated. The immunosuppressive combination therapy with ALG + Cyclosporin A + MTP represents a prospect.

Improved treatment results in childhood acute nonlymphoblastic leukemia with the BFM-AML protocol 78 in a multicenter study in the GDR.

Eighty-seven children with acute nonlymphoblastic leukemia were treated with the AML protocol BFM 78 between June 1979 and February 1986 in a multicenter study in the GDR. Seventeen children (20%) died from early complications, eight did not respond to therapy. Fifty-eight patients (70%) achieved a complete remission.

[Impulse cytophotometry studies of bone marrow and blood cells in children with acute lymphoblastic leukemia (ALL). 2. Lymphatic cells in blood].

The DNA-content of mononuclear cells of the peripheral blood of infantile and juvenile ALL patients was investigated using Pulse Cytophotometry. The fraction of cells in S- and G2 + M-phase is significantly increased in comparison with samples of healthy probands. The fraction of DNA-synthesising cells (S-phase) of both peripheral blood (mononuclear cells) and bone marrow of leukemia patients cannot be significantly distinguished by mathematical methods.

[Frequency distribution of DNA in blood and bone marrow cells of children with acute non-lymphatic leukemia using impulse cytophotometry].

1. As compared with the peripheral blood lymphocytes of healthy subjects the cells in the mononuclear fraction of peripheral blood and in the bone marrow of children with ANLL show a significant higher S- and G2 + M-phase. 2.

[Impulse cytophotometric studies of bone marrow and blood cells in children with acute lymphoblastic leukemia (ALL). 1. Bone marrow cells].

1. Compared with the peripheral blood lymphocytes of healthy children the cell fractions in the S- and G2 + M-phase are significantly higher in the bone-marrow of those children affected with ALL. This increase was proved in the SR- and MR-group irrespective of the cytomorphological subtype and cytochemical reaction.

[Side effects of therapy with antihuman lymphocyte globulin].

The most important side effects during AHLG-treatment of 34 children suffering from juvenile chronic arthritis, collagenoses, autoimmune chronic hepatitis, aplastic anaemia and acute lymphoblastic leukemia are: Thrombocytopenia, urticaria, fever, anaphylactic reaction. They all are reversible a short time after cessation of AHLG-administration.

[Bone marrow cytology in diseases of autoimmune pathogenesis in childhood and its changes during therapy with anti-human lymphocyte globulin (AHLG)].

Among the organs of the immune system the bone marrow is comparably simple accessible. Autoimmune diseases are characterized by reactive cytomorphological symptoms. Due to own investigations the granulo-erythropoietic index (G/E-index Rohr) as a parameter of inflammatory activity as well as the amount of plasma cells as an indication of humoral reactivity are described--before and under treatment with anti-human lymphocyte globulin.

[Suppression of lymphocyte proliferation in the blood of children with infectious mononucleosis. Flow cytometry study].

The investigation of lymphatic cells from venous blood shows an increase of cells in the S-phase during the acute stage of infectious mononucleosis, which is connected with a decrease of G1-phase cells. G2 + M fraction will remain within the normal range. These changes will completely recede within the first four weeks of the disease.

[Clinical effects of anti-human lymphocyte globulin in the treatment of collagenoses in childhood].

9 children with collagenoses were first treated with AHLG "Dessau" in combination with corticosteroids and then with Imurek (azathioprine). The children seemed to be vitally endangered and suffered from panarteritis, dermatomyositis, polyserositis or corticosteroid-resistent systemic juvenile rheumatoid arthritis. As assessed by decrease of inflammation and clinical symptoms the treatment appeared in one case to be very good and in another good; in four other children it was good for a short period; in three children there was no effect at all.

[Lymphocyte transformation using anti-lymphocyte globulin in child patients (author's transl)].

In normal children about under PHA 67% of the lymphocytes, but only 8% under AHLG show a blast transformation. Children with juvenile rheumatoid arthritis reveal (in autologeous serum) a PHA-reactivity of the normal range, whereas the transformation rate under AHLG is increased on more than 60%. In immune proliferative disease the results are different.

[Blood coagulation in newborns (author's transl)].

This article gives a survey of the physiology of blood coagulation in newborn infants, subdivided into the particularities of the plasmic coagulation system and the fibronolysis. Etiology, diagnosis and therapy of the consumption coagulopathy as well as the coagulopathy of production are dealed with.

[Possibilities for the treatment of varicella-herpes zoster infections in juvenile leukemia].

Chicken pox and herpes zoster represent afraid complications in patients with malignant diseases which may take a noxious course. During the sequence of two chicken pox epidemias in 1973 in children with acute lymphoblastic leukaemias, the prevention and therapeutic possibilities were checked again. Chicken pox or herpes zoster convalescent sera being compatible for blood groups and zoster-immunoglobulin were not available.