Improved cell growth on additively manufactured Ti64 substrates with varying porosity and nanofibrous coating.

3T3 Swiss albino mouse cells are often used in biotechnological applications. These cells can grow adherently on suitable surfaces. In our study, they were grown on different titanium substrates, comparing commercially available titanium sheets of grade 1 and grade 2, respectively, with Ti64 which was 3D printed with different porosity in order to identify potential substitutes for common well-plates, which could - in case of 3D printed substrates - be produced in various shapes and dimensions and thus broaden the range of substrates for cell growth in biotechnology and tissue engineering.