A New Expression System Based on Psychrotolerant Yeast and Its Application to the Production of Cold-Active β-d-Galactosidase from sp. 32d.

Yeasts provide attractive host/vector systems for heterologous gene expression. The currently used yeast-based expression platforms include mesophilic and thermotolerant species. A eukaryotic expression system working at low temperatures could be particularly useful for the production of thermolabile proteins and proteins that tend to form insoluble aggregates.