Preliminary Study on J-Resolved NMR Method Usability for Toxic Kidney's Injury Assessment.

Background: Nowadays, the Nuclear Magnetic Resonance (NMR) techniques are tested for metabolomic urine profile in order to detect early damage of kidney.

Objectives: The purpose of this investigation was the initial assessment of two-dimensional J-resolved NMR urine spectra analysis usability for early kidney injuries detection. The amino acids (AA) and acids profile change after the exposure to nephrotoxic agent (the cisplatin infusion) was examined.

Comparison of haptoglobin and alpha₁-acid glycoprotein glycosylation in the sera of small cell and non-small cell lung cancer patients.

Introduction: Cancer-related carbohydrate epitopes, which are regarded as potential diagnostic and prognostic biomarkers, are carried on the main acute phase proteins. It is not clear, however, if the glycosylation profile is similar in different glycoproteins, or it is protein specific to some extent. The aim of the study was to compare fucosylation, α2,3 sialylation and expression of sialyl-Lewisx epitopes (sLe(x)) in the serum as a whole, AGP and haptoglobin of small cell (SCLC) and non-small cell lung cancer (NSCLC) patients with respect to healthy subjects as well as the cancer stage and its histological type.

[Serum chitotriosidase activity as a biomarker of pulmonary sarcoidosis].

Sarcoidosis is a multisystemic granulomatous disorder of unknown etiology, which is characterized by a variable clinical presentation and course. The diagnosis of this disease is usually supported by the three elements: compatible clinical and radiologic findings, tissue biopsy specimen that reveals noncaseating epithelioid granulomas and the absence of known granulomagenic agent. During the last years the new diagnostic methods have been discovered, but serum markers of the sarcoidosis are still under studying.

CTLA-4, CD28, and ICOS gene polymorphism associations with non-small-cell lung cancer.

Polymorphisms in genes encoding CD28, ICOS, and CTLA-4 were demonstrated to be associated with susceptibility to malignancies. To the best of our knowledge, no study on this association has been performed in a Caucasian population for non-small-cell lung cancer (NSCLC). In the present work, we investigated the polymorphisms CTLA-4c.

Does the KIR2DS5 gene protect from some human diseases?

Background: KIR2DS5 gene encodes an activating natural killer cell receptor whose ligand is not known. It was recently reported to affect the outcome of hematopoietic stem cell transplantation.

Methodology/principal Findings: In our studies on KIR2DS5 gene associations with human diseases, we compared the frequencies of this gene in patients and relevant controls.

[Chronic obstructive pulmonary disease and cardiovascular diseases].

Chronic obstructive pulmonary disease (COPD) is a leading cause of morbidity and mortality worldwide. Patients with COPD are at increased risk of cardiovascular diseases, osteoporosis and lung cancer. Although some of the associations between COPD and atherosclerosis may be the result of common risk factors such as smoking, epidemiological evidence suggest that impaired lung function is a risk factor for increased cardiovascular death, independent of tobacco use.

[Pulmonary hypertension and pulmonary embolism in chronic obstructive pulmonary disease].

Chronic obstructive pulmonary disease (COPD) is a disease associated with high increasing worldwide mortality. COPD-related mortality is probably underestimated because of difficulties associated with identifying the precise cause of death. Respiratory failure is considered the major cause of death in advanced COPD.

[Serum activity of chitotriosidase, lysozyme and cathepsin H in patients with lung cancer and patients with inflammatory exudate (preliminary report)].

Unlabelled: Lung cancer remains the leading cause of cancer death over the world. Although new diagnostic methods have been discovered, new biomarkers of the cancer are still under studying. A human chitinolytic enzyme called chitotriosidase hydrolyzes chitin and chitotrioside substrates.

[A case of kappa L-chain primary nodular lung amyloidosis].

Amyloidosis is a generic term for a heterogeneous group of disorders associated with deposition of protein in an abnormal fibrillar form. We report a case of kappa L-chain primary lung amyloidosis. A 44-year-old woman without symptoms.

[The incidence and pathogenesis of cancer anorexia-cachexia syndrome in lung cancer].

The cachexia-anorexia syndrome (CACS) is common and important implication of cancer. It occurs in 30% to 80% cancer patients. At the time of diagnosis of lung cancer CACS is not yet very important problem, but the weight loss increases with progression of the cancer.

[Serum levels analysis of TGF-Beta and VEGF in patients with cancer anorexia-cachexia syndrome in lung cancer].

Unlabelled: The pathogenesis of cancer anorexia-cachexia syndrome (CACS) is very complicated. In development of this syndrome play a role: metabolism of glucose, tumor necrosis factor (TNF), interleukin 1, interleukin 6, interferon a, interferon y, the oncological treatment and many other factors. Vascular endothelial growth factor (VEGF) is the most important proangiogenic factor.

[Changes in the percentage of activated lymphocytes in bronchoalveolar lavage fluid in patients with clinical improvement of sarcoidosis].

Unlabelled: Sarcoidosis is characterized by an accumulation of the activated lymphocytes in affected organs. The aim of the study was the analysis of lymphocytes activation markers in a bronchoalveolar lavage fluid (BALF) in sarcoidosis and the influence of the disease outcome on the changes of cells subpopulations.

Material And Methods: We performed the study on 12 patients with the regression of sarcoidosis after the mean period of the observation 8.

Fucosylation of serum glycoproteins in lung cancer patients.

Increased expression of sialyl Lewis X or A antigens on metastatic cancer cells leads to their selectin-mediated extravasation. Profound fucosylation of the serum microenvironment may be a factor that interrupts adhesion and influences the formation of metastases. In this study we quantitatively analyzed fucosylation of serum glycoproteins in small-cell and non-small-cell lung cancer patients.

Skin reactivity to histamine and expression of histamine receptors mRNA in lymphocytes of healthy subjects and non-small-cell lung cancer patients before and after surgery.

Histamine modulates an immunological response through stimulation of appropriate receptor--H1R proinflammatory or H2R suppressive. The participation of histamine in regulation of an immunological response in the course of neoplastic disease is determined by the expression of particular receptor. The aim of our work was the investigation of the expression of mRNA of two types of histamine receptors in peripheral blood lymphocytes and the evaluation of skin-prick test with histamine in lung cancer patients before and after surgery.

[Cathepsin B activity and concentration of elastase and alpha-1 proteinase inhibitor complex in non-small-cell lung cancer: 2 year follow-up study].

In 21 patients with non-small-cell lung cancer subjected to radical surgery followed by 3 cycles of chemotherapy, serum cathepsin B activity and plasma E-alpha 1IP concentration in peripheral blood and tumour arterial and venous blood were studied. Cathepsin B activity was determined by a fluorometric assay. E-alpha 1IP concentration was measured with an ELISA kit.

[Elastase and alpha-1 proteinase inhibitor complex in broncho-alveolar lavage fluid in patients with bronchial asthma and sarcoidosis].

Forty-three patients were enrolled into this study, twenty-eight with sarcoidosis in various radiological stages (I, II, III) and activity of disease (active or non-active) and fifteen with bronchial asthma. Elastase and alpha 1-proteinase inhibitor complex (E-alpha 1IP) concentrations and percentage of neutrophils in broncho-alveolar lavage fluid (BALF) were measured in all groups of patients. There were no significant differences in E-alpha 1IP levels in patients with various activity of sarcoidosis.

[Serum and bronchoalveolar lavage fluid neopterin levels in patients with bronchial asthma].

Neopterin is an activation marker of macrophages. These cells become activated in bronchial asthma. The aim of the study was to measure serum and BALF neopterin levels in patients with bronchial asthma.

[Increase of skin reactivity to histamine in patients after lung cancer surgery].

In 16 patients with lung cancer submitted to radical surgery skin reactivity was studied. Skin prick test was done in all patients with histamine concentration--10 mg/ml and negative control before and after surgery. The longest wheal diameter was measured after 15 min and 30 min.

[Soluble interleukin 2 receptor (SIL-2R) in bronchoalveolar lavage fluid (BALF) of patients with sarcoidosis and atopic bronchial asthma].

The aim of the study was assessment of sIL-2R in BALF in asthma and sarcoidosis patients. Forty three patients were enrolled in this study. Twenty eight with sarcoidosis (S) in different stages (I, II, III) and activity of disease: active (AS) or nonactive (NAS) and fifteen with atopic bronchial asthma (AO).

[Tumor markers TPA and Cyfra 21.1 in patients with non-small cell lung cancer after surgery and chemotherapy].

In 27 patients with operable non-small cell lung cancer (NSCLC) submitted to radical surgery followed by 3 cycles of chemotherapy (cht) serum concentrations of Cyfra 21.1 and TPA were studied. The measurements were performed before and 14 days after surgery, before each cht and every 60th day after cht was completed, for 2 years.

[Anticancer chemotherapy containing cisplatin in patients with lung cancer and kidney function].

To carry out safe anticancer chemotherapy one should consider the kidney function. Insufficiency of that main organ responsible for drugs excretion, caused either by neoplastic disease or by chemotherapy, can diminish the possibility or even make impossible of carrying out a complete treatment cycle. The aim of our work was to evaluate the kidney function in patients with lung cancer during anticancer chemotherapy containing cisplatin.

[The effect of inhaled low molecular weight heparin on cell composition in bronchoalveolar lavage fluid and serum levels of soluble receptor IL-2 in bronchial asthma patients].

The 17 asthmatics were studied. The patients were administered LMWH inhalations during two weeks in a day dose 5000 IU anti-Xa. The cell composition in BALF and serum levels of sIL-2R were estimated before and two weeks after treatment.