Application of ion chromatography with pulsed amperometric detection for the determination of trace cyanide in biological samples, including breast milk.

Cyanide (CN) is the biomarker of exposure to the components of tobacco smoke, although its presence in biological samples is also due to the consumption of products containing cyanogenic glycosides. In this work, we determine the concentration of the free cyanide in urine, saliva and breast milk matrices, using ion chromatography with pulsed amperometric detection (IC-PAD). IC-PAD is an emerging method, with only few documented applications in urine and saliva, and the presented determination in breast milk is its first published report for any method.

Development of an Analytical Protocol for Determination of Cyanide in Human Biological Samples Based on Application of Ion Chromatography with Pulsed Amperometric Detection.

A simple and accurate ion chromatography (IC) method with pulsed amperometric detection (PAD) was proposed for the determination of cyanide ion in urine, sweat, and saliva samples. The sample pretreatment relies on alkaline digestion and application of Dionex OnGuard II H cartridge. Under the optimized conditions, the method showed good linearity in the range of 1-100 g/L for urine, 5-100 g/L for saliva, and 3-100 g/L for sweat samples with determination coefficients () > 0.

Determination of thiocyanate as a biomarker of tobacco smoke constituents in selected biological materials of human origin.

In order to protect human health, it is necessary to biomonitor toxic substances originating from tobacco smoke in biological materials sampled from persons with different exposures to tobacco smoke constituents. Thiocyanate anion is a biomarker of exposure to tobacco smoke components which is characterized by a relatively long half-life in the human body, i.e.

Determination of cyanide in urine and saliva samples by ion chromatography with pulsed amperometric detection.

Abstract: Commonly known as a highly toxic chemical, cyanide is also an essential reagent for many industrial processes. It naturally occurs in plant seeds as cyanogenic glycosides. Another relatively common mode of cyanide exposure is inhalation of environmental tobacco smoke.

Cyanides in the environment-analysis-problems and challenges.

Cyanide toxicity and their environmental impact are well known. Nevertheless, they are still used in the mining, galvanic and chemical industries. As a result of industrial activities, cyanides are released in various forms to all elements of the environment.