Publications by authors named "Evsev'eva A"

Cytochemical demonstration of levamisole-sensitive alkaline phosphatase (AP) have been used for the identification of tumor cells in serous effusions. AP-activity is not demonstrable in mesothelial cells and histiocytes-macrophages in serous effusions in 18 noncancer patients. Enzyme activity is detected in cancer cells in serous effusions in 19 from 52 cancer patients.

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Pleural cavity exudates obtained from 26 patients with cancer of the lung, ovary, stomach and breast and 13 cases of nononcological pathologies were studied using enzymatic cytochemical methods and monoclonal antibodies. A complex of tissue markers (carcinoembryonic antigen, 90 kdalton glycoproteid, activity of alkaline phosphatase and nonspecific alpha-naphthylacetate esterase, and PAS-positive bodies) was identified. It allows to differentiate between malignant and mesothelial cells in smears prepared from exudates.

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Spleen cells from BALB/c mice previously immunized with B-lymphoblastoid cell line RPMI-1788 were fused with P3-X-63-Ag8.653 myeloma cells. Monoclonal antibodies (Ab) IPO-4 were screened on 18 cell lines by the indirect immuno-fluorescence method.

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Data are reported of a study of the immunophenotypic aspects of lymphocytes in exudates from the pleural cavity realized by means of an immunoperoxidase method using monoclonal antibodies produced in the USSR. The importance is shown of the investigation for the differential diagnosis of lymphoid reactions and B-lymphoproliferative diseases.

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Methods of monoclonal antibodies, cytochemistry and rosette formation have been used to study antigens, receptors and enzymatic activity of peripheral blood lymphocytes during pokeweed mitogen-stimulation. After 18-20 hours in mitogen-stimulated cultures there is a decrease in number of T-lymphocytes that express CD7, CD5, CD4, CD8 antigens and of E-receptors and cells with the local activities of acid phosphatase and acid non-specific esterase. The number of lymphocytes with E37-, FcM- and M-receptors and of cells with granular PAS-reaction increased.

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A method for the determination of surface proteins and cellular elements cytoskeleton proteins in exudates from the pleural cavity is described. Use of this method has helped identify carcinoma cells in exudates of 19 patients out of the 21 examinees with oncologic diseases and permitted a characterization of the subpopulation composition of lymphocytes. This method may be employed as an additional test for the detection of carcinoma cells in exudates from serous cavities.

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Monoclonal antibodies IPO-10 were generated following immunization of a BALB/c mice with human cell line Daudi. Reactivity of this mABs was studied by indirect immunofluorescence technique with 10 human cell lines, blood cells of healthy persons and of patients with the malignant lymphoproliferative diseases. Studies on normal and neoplastic B cell suggest that mABs IPO-10 recognizing antigen is B lineage restricted.

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Surface phenotype of peripheral blood lymphocytes was studied in 18 patients with myeloma by combined rosette-forming and immunofluorescent methods. A high content of B lymphocytes with the identical type of immunoglobulin, and a decreased level of T lymphocytes were revealed. The increased activity of spontaneous and Con A-induced suppressor activity and cytochemical changes of some intracellular hydrolytic enzymes were found in lymphocytes of patients with myeloma.

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The dynamics of cell-mediated immunity indices in 83 patients with rectal tumors who underwent surgery or received combined treatment (preoperative radiation + surgery) was followed. Cellular immunity was assessed on the basis of PHA-induced blastogenic reaction of lymphocytes (BRL) and spontaneous rosette-formation. In rectal tumor patients, particularly at stage IV, the level of PHA-induced BRL and the total number of rosette-forming cells (RFC) were lower than in healthy donors.

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A study was made of the PHA-induced lymphocyte blastransformation (BT), and lymphocyte antitumor activity, using a model of progressive and regressive neoplastic process induced by the Moloney virus in mice BALB/c. The antitumor acitvity of lymphocytes was estimated by their ability to inhibit spheroid production by tumor cells. A lymphocyte BT and their antitumor activity was registered during a progressive growth of the tumor and its restoration as the tumor is regressing.

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An extensive surgical intervention in removing a tumor-bearing limb induce in mice an enhancement of immunosuppression state, developed in the process of carcinogenesis. Rehabilitation of the immunocompetence in such mice proceeds in a more active way than in the control animals, subjected to the analogous amputation of the extremity.

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