Publications by authors named "Evgeny Degtyaryov"

Article Synopsis
  • A transformation protocol using dual selection (fluorescent protein and herbicide resistance) was applied to create transgenic emmer and bread wheat plants aimed at increasing the production of the stress hormone jasmonates through gene overexpression.
  • The gene expression affected the growth negatively, leading to issues like reduced leaf length and fewer grains per spike in both wheat species, with a particularly severe impact on emmer wheat.
  • Among the genes tested, overexpressing one specific gene significantly enhanced levels of jasmonates, particularly in emmer wheat, but also brought about detrimental effects on plant size and reproductive success.
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12-Oxophytodienoate reductase is the enzyme involved in the biosynthesis of phytohormone jasmonates, which are considered to be the major regulators of plant tolerance to biotic challenges, especially necrotrophic pathogens. However, we observe compromised tolerance to the necrotrophic fungal pathogen in transgenic hexaploid bread wheat and tetraploid emmer wheat plants overexpressing gene from , while in Arabidopsis plants themselves, endogenously produced and exogenously applied jasmonates exert a strong protective effect against . Exogenous application of methyl jasmonate on hexaploid and tetraploid wheat leaves suppresses tolerance to and induces the formation of chlorotic damages.

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For immobile plants, the main means of protection against adverse environmental factors is the biosynthesis of various secondary (specialized) metabolites. The extreme diversity and high biological activity of these metabolites determine the researchers' interest in plants as a source of therapeutic agents. Oxylipins, oxygenated derivatives of fatty acids, are particularly promising in this regard.

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