Publications by authors named "Evgeniya Y Kirichenko"
Article Synopsis
- - Gap junctions are channels that allow communication between adjacent cells, but their role in brain tumors, particularly glioblastoma multiforme (GBM), is not well understood.
- - Research shows that the protein Connexin 43 (Cx43) and the polyamine spermine are co-expressed in human GBM samples, implying they might work together in tumor development and integration into surrounding neural networks.
- - The study utilized various microscopy techniques to demonstrate that Cx43 and spermine localize in specific regions of GBM, indicating that they may facilitate gap junction activity, which could enable tumor cells to share metabolic products with neighboring cells.
Article Synopsis
- - Connexin 43 (Cx43) is a multifunctional protein important for gap junction communication and is thought to influence the behavior of anaplastic astrocytomas during tumor growth through mechanisms like neuroinflammation and oxygen-glucose deprivation.
- - Researchers studied Cx43 localization and gap junction ultrastructure in malignant brain tumors by using confocal laser microscopy and electron microscopy on tumor samples, specifically looking at the interactions between Cx43 and GFAP (a glial protein).
- - Their findings showed that Cx43 was prominently present in the tumor cells, indicating many gap junctions and suggesting that these connections might facilitate communication and metabolic exchange between glioma cells, potentially aiding in tumor progression.
Fish as model objects have found wide applications in biology and fundamental medicine and allow studies of behavioral and physiological responses to various environmental factors. Representatives of the genus are one of the most convenient objects for such studies. Male fish belonging to the family are characterized by extremely diverse coloration, which constantly changes, depending on the age of the fish, environmental factors, and social hierarchical status.
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- Current assessment methods for penile cavernous fibrosis in animal models are limited, prompting the study to evaluate micro-computed tomography (micro-CT) as a more effective alternative.
- The study used 25 male rabbits with induced testosterone deficiency, assessing changes in penile samples over a period of 84 days through various analysis methods, including 3D modeling and tissue morphometry.
- Results indicated that micro-CT provided detailed visualization and changes in tissue density, with significant alterations in smooth muscle/connective tissue and collagen ratios observed at different time points, suggesting a relationship between gray value changes and tissue reorganization in fibrosis.
The phenomenon of unstable expression of gap junction's proteins connexins remains a "visiting card" of astrocytic tumors with various degrees of malignancy. At the same time, it stays unclear what is detected by the positive expression of connexins in astrocytic tumors: gap junctions, hemi-channels, or connexin proteins in cytosol. In the present work, for the first time, we demonstrate an ultrastructural evidence of gap junctions in pleomorphic xanthoastrocytoma, a rare primary brain tumor, the intercellular characteristics of which are poorly studied and remain very discursive and controversial.
View Article and Find Full Text PDFLaminar distribution of gap-junctions in rat somatic cortical columns.
J Integr Neurosci
December 2009
Results about the spindle-like activity peculiarities and the dendro-dendritic gap-junctions numbers in the infragranular and supragranular layers (modules) of the rat somatic cortex columns are presented. During simultaneous recording of extracellular field potentials from the cortical columns, the following was revealed: (i) the spindle-like activity was more synchronized inside one cortical column than in different columns; (ii) in most of the experiments (9 out of 10) an amplitude of the spindle waves was two times more in the supragranular layers than in the infragranular layers. During electron microscopy investigation of the experimental objects used in the electrophysiological study, it was shown that the number of the gap-junctions was more in the supragranular columnar layers as compared with the infragranular layers (4.
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