Ground Rules for Preschooler Exposure to the Digital Environment: A Review of Studies.

Background: The range of digital technologies that children use from an early age has expanded significantly. Most studies demonstrate that preschoolers now spend substantially longer on digital devices and start using them at a younger age. Finding a solution for this challenge has research merits and relevance, as the data on benefits and harm of early preschoolers' exposure to digital devices is contradictory.

Diastereoselective synthesis of tetrahydropyrrolo[1,2-]oxadiazoles from functionalized Δ-pyrrolines and generated nitrile oxides.

Tetrahydropyrrolo[1,2-]oxadiazoles have been synthesized in good-to-excellent yields the cycloaddition of nitrile oxides ( generated from aldoximes) to readily accessible functionalized Δ-pyrrolines. The reaction proceeds smoothly at room temperature in a two-phase system in the presence of sodium hypochloride as an oxidant to diastereoselectively afford pharmaceutically prospective 1,2,4-oxadiazolines fused with a five-membered ring. The reaction tolerates a broad range of substrates, including those with oxidant-sensitive functional groups and competitive reaction sites.

Hydrazides in the reaction with hydroxypyrrolines: less nucleophilicity - more diversity.

Expedient protocols for the synthesis of three types of highly functionalized azaheterocyclic scaffolds (dihydropyridazines, tetrahydropyridazines, and partially saturated tricyclic systems) from readily available hydroxypyrrolines and hydrazides are described. The directions of the transformation of a common initial intermediate, namely a Brønsted acid-activated hydroxypyrroline, depend on the reaction conditions and the structure of the hydrazides.

Authoritative subspecies diagnosis tool for European honey bees based on ancestry informative SNPs.

Background: With numerous endemic subspecies representing four of its five evolutionary lineages, Europe holds a large fraction of Apis mellifera genetic diversity. This diversity and the natural distribution range have been altered by anthropogenic factors. The conservation of this natural heritage relies on the availability of accurate tools for subspecies diagnosis.

Recessive myotonia congenita caused by a homozygous splice site variant in CLCN1 gene: a case report.

Background: Myotonia congenita is a rare neuromuscular disease, which is characterized by a delay in muscle relaxation after evoked or voluntary contraction. Myotonia congenita can be inherited in a dominant (Thomsen disease) and recessive form (Becker disease) and both are caused by pathogenic variants in the CLCN1 gene. Noncanonical splice site variants are often classified as variants of uncertain significance, due to insufficient accuracy of splice-predicting tools.

Pathomechanisms of a Mutation Found in a Russian Family Suffering From Becker's Myotonia.

Myotonia congenita (MC) is a rare muscle disease characterized by sarcolemma over-excitability inducing skeletal muscle stiffness. It can be inherited either as an autosomal dominant (Thomsen's disease) or an autosomal recessive (Becker's disease) trait. Both types are caused by loss-of-function mutations in the gene, encoding for ClC-1 chloride channel.

Efficacy of covert closed-circuit television monitoring of the hand hygiene compliance of health care workers caring for patients infected with multidrug-resistant organisms in an intensive care unit.

Background: To compare covert closed-circuit television (CCTV) monitoring to standard overt observation in assessing the hand hygiene (HH) conduct of health care workers (HCWs) caring for patients infected with multidrug-resistant organisms (MDROs). This was a cross-sectional study in a general intensive care unit of a 1,000-bed university hospital.

Methods: Forty-six general intensive care unit HCWs (staff physicians, registered nurses, and auxiliary workers) caring for contact isolation MDRO-infected patients.

Allozyme polymorphism and phylogenetic relationships in Apis mellifera subspecies selectively reared in Poland and Bulgaria.

The genetic variability of honey bee populations of three subspecies selectively reared in Poland (A. m. carnica and A.