Publications by authors named "Evgeniy A Pokushalov"
Properties of matrices manufactured by electrospinning from solutions of polyurethane Tecoflex EG-80A with gelatin in 1,1,1,3,3,3-hexafluoroisopropanol were studied. The concentration of gelatin added to the electrospinning solution was shown to influence the mechanical properties of matrices: the dependence of matrix tensile strength on protein concentration is described by a bell-shaped curve and an increase in gelatin concentration added to the elasticity of the samples. SEM, FTIR spectroscopy, and mechanical testing demonstrate that incubation of matrices in phosphate buffer changes the structure of the fibers and alters the polyurethane-gelatin interactions, increasing matrix durability.
View Article and Find Full Text PDFMicroRNAs (miRNAs) constitute a class of small noncoding RNAs that plays an important role in the post-transcriptional regulation of gene expression. Much evidence has demonstrated that miRNAs are involved in regulating the human and mouse pluripotency. Nevertheless, to our knowledge, miRNAs in the pluripotent stem cells of one of the most commonly used model organisms - the Rattus norvegicus have not been studied.
View Article and Find Full Text PDFArticle Synopsis
- Rat pluripotent stem cells, including embryonic (ESCs) and induced pluripotent stem cells (iPSCs), show promise for research on early mammalian development, but data on rat pluripotency and differentiation methods are limited.
- The study utilized RNA-Seq to analyze the transcriptomes of rat ESCs and iPSCs, revealing that they are quite similar and distinct from differentiated cells, along with a notable X-chromosome reactivation during reprogramming.
- A new monolayer differentiation protocol was developed to better understand X-chromosome inactivation dynamics and facilitate the creation of specific cell types for biomedical applications.