Paediatric pancreatic trauma in North Queensland: a 10-year retrospective review.

Purpose: To establish the incidence of pancreatic trauma in North Queensland to the region's only tertiary paediatric referral centre, and to determine the patient's outcomes based on their management.

Methods: A single centre, retrospective cohort study of patients < 18 years with pancreatic trauma from 2009 to 2020 was performed. There were no exclusion criteria.

Youth Exposure to Marijuana Advertising in Oregon's Legal Retail Marijuana Market.

Introduction: Research from tobacco and alcohol markets suggests advertising exposure is associated with perceptions of lower risk and increased use among young people. Limiting marketing may be a regulatory approach to prevent potential negative effects of retail marijuana legalization on youth use. This study assessed marijuana advertising exposure reported by youths in Oregon after the start of retail marijuana sales in October 2015.

Post-Legalization Opening of Retail Cannabis Stores and Adult Cannabis Use in Washington State, 2009-2016.

To assess the relationship between adult cannabis use and time-varying local measures of retail cannabis market presence before and after legalization (2012) and market opening (2014) in Washington State. We used 2009 to 2016 data on 85 135 adults' current (any) and frequent (20 or more days) past-month cannabis use from the Washington Behavioral Risk Factor Surveillance System linked to local retailer proximity and density. Multilevel models predicted use over time, accounting for nesting within communities.

Efficacy and safety of a clinically relevant foamy vector design in human hematopoietic repopulating cells.

Background: Previous studies have shown that foamy viral (FV) vectors are a promising alternative to gammaretroviral and lentiviral vectors and also that insulators can improve FV vector safety. However, in a previous analysis of insulator effects on FV vector safety, strong viral promoters were used to elicit genotoxic events. In the present study, we developed and analyzed the efficacy and safety of a high-titer, clinically relevant FV vector driven by the housekeeping promoter elongation factor-1α and insulated with an enhancer blocking A1 insulator (FV-EGW-A1).

Evidence for the in vivo safety of insulated foamy viral vectors.

Retroviral vector-mediated stem cell gene therapy is a promising approach for the treatment of hematopoietic disorders. However, genotoxic side effects from integrated vector proviruses are a significant concern for the use of retroviral vectors in the clinic. Insulated foamy viral (FV) vectors are potentially safer retroviral vectors for hematopoietic stem cell gene therapy.

Spatially resolved Thomson scattering measurements of the transition from the collective to the non-collective regime in a laser-produced plasma.

We present optical Thomson scattering results that image for the first time in a single measurement the spatial transition from collective to non-collective scattering. Data were taken in the Phoenix laser laboratory at the University of California, Los Angeles. The Raptor laser was used to ablate a carbon plasma, which was diagnosed with the frequency-doubled Phoenix laser serving as a Thomson scattering probe.

A comparison of foamy and lentiviral vector genotoxicity in SCID-repopulating cells shows foamy vectors are less prone to clonal dominance.

Hematopoietic stem cell (HSC) gene therapy using retroviral vectors has immense potential, but vector-mediated genotoxicity limits use in the clinic. Lentiviral vectors are less genotoxic than gammaretroviral vectors and have become the vector of choice in clinical trials. Foamy retroviral vectors have a promising integration profile and are less prone to read-through transcription than gammaretroviral or lentiviral vectors.

Retroviral vector interactions with hematopoietic cells.

Hematopoietic stem cell (HSC) gene therapy using retroviral vectors is a powerful and promising approach to permanently correct many hematopoietic disorders. Increasing the transduction of quiescent HSCs and reducing genotoxicity are major challenges in the field. Retroviral vectors, including lentiviral and foamy vectors, have been extensively modified resulting in improved safety and efficacy.

Estimating the Prevalence of Childhood Obesity in Alaska Using Partial, Nonrandom Measurement Data.

Although monitoring childhood obesity prevalence is critical for state public health programs to assess trends and the effectiveness of interventions, few states have comprehensive body mass index measurement systems in place. In some states, however, assorted school districts collect measurements on student height and weight as part of annual health screenings. To estimate childhood obesity prevalence in Alaska, we created a logistic regression model using such annual measurements along with public data on demographics and socioeconomic status.

Enhanced collisionless shock formation in a magnetized plasma containing a density gradient.

Two-dimensional hybrid simulations of super-Alfvénic expanding debris plasma interacting with an inhomogeneous ambient plasma are presented. The simulations demonstrate improved collisionless coupling of energy to the ambient ions when encountering a density gradient. Simulations of an expanding cylinder running into a step function gradient are performed and compared to a simple analytical theory.

Identification of the docking site between a type III secretion system ATPase and a chaperone for effector cargo.

A number of Gram-negative pathogens utilize type III secretion systems (T3SSs) to inject bacterial effector proteins into the host. An important component of T3SSs is a conserved ATPase that captures chaperone-effector complexes and energizes their dissociation to facilitate effector translocation. To date, there has been limited work characterizing the chaperone-T3SS ATPase interaction despite it being a fundamental aspect of T3SS function.

Modulation of antigenic location converts chronic into acute infection by forcing CD8+ T cell recognition.

Pathogens that reside in the phagosomes of infected cells persist despite the presence of potent T cell responses. We addressed the mechanism of immune evasion by using a mouse model of Salmonella typhimurium (ST). Recombinants of ST were generated that translocated antigen to the cytosol or phagosomes of infected cells.

Feasibility of characterizing laser-ablated carbon plasmas via planar laser induced fluorescence.

Planar laser induced fluorescence (PLIF) imaging can potentially assess ion distributions and coupling in the context of super-Alfvénic ablation plasma expansions into magnetized background plasmas. In this feasibility study, we consider the application of PLIF to rapidly expanding carbon plasmas generated via energetic laser ablation of graphite. By utilizing hydrodynamic and collisional-radiative simulations, we identify schemes accessible to commercially available tunable lasers for the C I atom, the C II ion, and the C V ion.

Magnetic field measurements in low density plasmas using paramagnetic Faraday rotator glass.

Paramagnetic Faraday rotator glass (rare-earth doped borosilicate) with a high Verdet constant will be used to measure the magnetic field inside of low density Helium plasmas (T(e) ~ 5 eV, T(i) ~ 1 eV) with a density of n ~ 10(12) cm(-3). Linearly polarized light is sent through the glass such that the plane of polarization is rotated by an angle that depends on the strength of the magnetic field in the direction of propagation and the length of the crystal (6 mm). The light is then passed into an analyzer and photo-detector setup to determine the change in polarization angle.

Generation of scaled protogalactic seed magnetic fields in laser-produced shock waves.

The standard model for the origin of galactic magnetic fields is through the amplification of seed fields via dynamo or turbulent processes to the level consistent with present observations. Although other mechanisms may also operate, currents from misaligned pressure and temperature gradients (the Biermann battery process) inevitably accompany the formation of galaxies in the absence of a primordial field. Driven by geometrical asymmetries in shocks associated with the collapse of protogalactic structures, the Biermann battery is believed to generate tiny seed fields to a level of about 10(-21) gauss (refs 7, 8).

Clients' perspectives on therapy termination.

We used consensual qualitative research to analyze interviews with 12 clients about their termination from psychotherapy. Those who had positive termination experiences reported a strong therapeutic relationship and positive outcomes of therapy. They terminated primarily for logistical or financial reasons; their termination, post-termination plans, and feelings about termination were discussed in advance with their therapist, as was their growth in therapy, leading to mostly positive effects of the termination.

A scalable multipass laser cavity based on injection by frequency conversion for noncollective Thomson scattering.

A scalable setup using injection by frequency conversion to establish a multipassing cavity for noncollective Thomson scattering on low density plasmas is presented. The cavity is shown to support >10 passes through the target volume with a 400% increase in energy on target versus a single-pass setup. Rayleigh scattering experiments were performed and demonstrate the viability of the cell to study low density plasmas of the order of 10(12)-10(13) cm(-3).

Additive value of nuclear medicine shuntograms to computed tomography for suspected cerebrospinal fluid shunt obstruction in the pediatric emergency department.

Objective: To measure the predictive value of nuclear medicine studies (cerebrospinal fluid [CSF] shuntograms) and radiographic studies (computed tomographic [CT] scans) in a cohort of children undergoing evaluation for suspected shunt obstruction in a tertiary care pediatric emergency department (ED).

Methods: A retrospective chart review was conducted on patients younger than 18 years who presented to the pediatric ED of the Children's Hospital of Western Ontario and had both CT of the head and a CSF shuntogram ordered by the attending pediatric emergency medicine physician between December 1998 and April 2003 because of suspected shunt obstruction.

Results: A total of 69 patients were evaluated for suspected shunt obstruction in the ED during this period with both a CT and a CSF shuntogram.

Design, construction, and calibration of a three-axis, high-frequency magnetic probe (B-dot probe) as a diagnostic for exploding plasmas.

A three-axis, 2.5 mm overall diameter differential magnetic probe (also known as B-dot probe) is discussed in detail from its design and construction to its calibration and use as diagnostic of fast transient effects in exploding plasmas. A design and construction method is presented as a means to reduce stray pickup, eliminate electrostatic pickup, reduce physical size, and increase magnetic signals while maintaining a high bandwidth.

Determinants of physical activity promotion by smoking cessation advisors as an aid for quitting: support for the Transtheoretical Model.

Objectives: Physical activity (PA) can reduce cigarette cravings and aid quitting but little is known about its promotion by smoking cessation advisors. This study aimed to: (1) determine the extent to which smoking cessation advisors promote PA; and (2) examine the relationship between PA promotion as a cessation aid and advisor characteristics and cognitions, within the Transtheoretical Model (TM) framework.

Methods: Self-report surveys assessing PA promotion, TM variables, advisors' own PA levels and demographics were completed by 170 advisors in England and Scotland.

Brief report: the theory of planned behaviour applied to physical activity in young people who smoke.

It has been hypothesised that physical activity may be useful as a smoking cessation intervention for young adults. In order to inform such interventions, this study evaluated the theory of planned behaviour (TPB) for understanding physical activity behaviour in young smokers. Regular smokers aged 16-19 years (N=124), self-reported physical activity and all TPB components.

Does exercise have an acute effect on desire to smoke, mood and withdrawal symptoms in abstaining adolescent smokers?

Objective: Previous studies have shown that exercise acutely reduces desire to smoke and withdrawal symptoms among adult smokers; however, no study has examined these effects in younger smokers. This study investigated the impact of a short bout of moderate intensity exercise on desire to smoke, withdrawal symptoms and exercise-induced affect in temporarily abstinent adolescent smokers.

Methods: Thirty-seven low-active male and female smokers aged 16-19 years, abstained from smoking overnight and were assigned to either (i) 10 min of moderate intensity cycle ergometry (n=18) or (ii) a placebo control condition that involved very light intensity cycle ergometry (n=19).

Predictors of general surgical complications after heart transplantation.

Background: Several studies have reported the incidence, morbidity, and mortality of general surgical conditions (GSCs) in orthotopic heart transplant (OHT) patients. The following is the largest reported series of such patients and the first study with sufficient patient numbers to formally evaluate peritransplant variables as risk factors for GSC development.

Study Design: A GSC was defined as a condition for which a general surgeon had been consulted or as a general surgical condition recognized at the time of autopsy.

Cold acclimation in Arabidopsis and wheat : a response associated with expression of related genes encoding ;boiling-stable' polypeptides.

Changes in gene expression occur during cold acclimation in a wide variety of plant species. Here we show that a number of the polypeptides encoded by cold-regulated (cor) genes of Arabidopsis thaliana L. (Heyn) and wheat share the unusual biochemical property that they remain soluble upon boiling in aqueous solution.