Publications by authors named "Evelyne Ng Kwan Lim"
Article Synopsis
- Iron is crucial for various metabolic processes, and a lack of it can hinder cell division; however, the specific mechanism for this was not well understood.
- Researchers identified that the small RNA RyhB interacts with mRNA responsible for chromosome segregation and cell division, repressing its translation during iron shortage.
- Experiments showed that without RyhB, bacterial cells grew longer and had issues with chromosome separation, suggesting that RyhB plays a critical role in adapting cell division processes in response to iron availability.
The operon of Qin cryptic prophage in Escherichia coli K-12 encodes the small RNA (sRNA) DicF and small protein DicB, which regulate host cell division and are toxic when overexpressed. While new functions of DicB and DicF have been identified in recent years, the mechanisms controlling the expression of the operon have remained unclear. Transcription from the major promoter of the operon, is repressed by DicA.
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- * The translation process is influenced by specific elements on RNA transcripts that interact with regulators, including small regulatory RNAs (sRNAs), which help control how and when translation starts.
- * The text highlights examples of how these sRNA interactions work and discusses the potential for sRNAs to uncover new functions of RNA elements, while also mentioning the challenges of utilizing translation regulation in synthetic biology.
RNA binding proteins (RBPs) are ubiquitously found in all kingdoms of life. They are involved in a plethora of regulatory events, ranging from direct regulation of gene expression to guiding modification of RNA molecules. As bacterial regulators, RBPs can act alone or in concert with RNA-based regulators, such as small regulatory RNAs (sRNAs), riboswitches, or clustered regularly interspaced short palindromic repeats (CRISPR) RNAs.
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