Apple proanthocyanidins do not reduce the induction of preneoplastic lesions in the colon of rats associated with human microbiota.

Since the gut microbiota metabolizes various dietary constituents unabsorbed by the small intestine and modulates colon function, it plays an essential role in colon carcinogenesis. First, we have developed a model of human microbiota-associated rats (HMA), fed a human-type diet and injected with 1-2,dimethylhydrazine (DMH). We observed that the number and size of DMH-induced aberrant crypt foci (ACF) were significantly higher in HMA rats than in germ-free or conventional rats.

Brussels sprouts, inulin and fermented milk alter the faecal microbiota of human microbiota-associated rats as shown by PCR-temporal temperature gradient gel electrophoresis using universal, Lactobacillus and Bifidobacterium 16S rRNA gene primers.

We investigated the effect of Brussels sprouts, inulin and a fermented milk on the faecal microbiota diversity of human microbiota-associated (HMA) rats by PCR-temporal temperature gradient gel electrophoresis (PCR-TTGE) using universal and group-specific 16S rRNA gene primers. The HMA rats were submitted to a control diet for 10 d (initial time), then switched to the experimental diets for 4 weeks (final time). Using universal primers, the mean degree of similarity between all faecal samples at initial time was 80.

Administration of two antagonists of the cholecystokinin(B)/gastrin receptor does not totally inhibit the pancreatic response to a meal in the pig.

Introduction And Aims: The role of the cholecystokinin B (CCK(B))/gastrin receptor in the pancreatic response to a standard meal was investigated in the pig.

Methodology: Twenty-four pigs were prepared surgically for the collection of the pancreatic juice and an intravenous perfusion. On experimental days, the pigs were perfused with one of two CCK(B)antagonists (L-365,260 or PD 135156) or the vehicle for 2 hours.