Droplet digital PCR versus real-time PCR for in-house validation of porcine detection and quantification protocol: An artificial recombinant plasmid approach.

Authenticity and traceability are essential for modern food and medicine inspection, and reliable techniques are important for the trade of halal foods, which reach more than 20 percent of the world market. A sensitive and accurate porcine detection method is required to develop a conformity assessment system that includes laboratory testing for porcine-free certification. This study proposes a procedure that could be incorporated into the development of a standardized control and protocol for real-time PCR (qPCR) methods and their traceability using droplet digital PCR (ddPCR).

Effect of environmental factors on blood counts of caught at Brantas River watershed, Indonesia.

: Contamination of freshwater ecosystems has become a major issue as it threatens public water sources as well as aquatic life. It is important to predict changes in organism health, given a known number of environmental factors and pollutant concentrations, in order to better manage contaminants through biomarker analysis. This study aims to examine the ecosystem health of the Brantas River based on its environmental condition and the hematology profile of fish present in the river.