Background: Pathological concentrations of plasma proteins may confound the results of binding assays. We compared two routinely used total 25-hydroxyvitamin D [t-25(OH)D] methods: a chemiluminescence-immuno-(CLIA) and an electro-chemiluminescence-protein-binding-(ECLPBA) assay.
Methods: Two sub-studies were performed: 1) In an "in vitro" study, exogenous albumin was added to pools of patients' sera with low albumin levels; and 2) In "ex vivo" studies of Cohort_1: sera of hospitalized patients with low albumin levels, and of healthy controls; and of Cohort_2: outpatients with chronic kidney disease in pre-dialysis stage, or on peritoneal dialysis and hemodialysis were investigated by the routine and LC_MS/MS methods.
Introduction: Parathyroid hormone levels provide important information in chronic renal failure.
Aim: To compare parathyroid hormone levels measured by two assays in correlation with vitamin D supply.
Method: Parathyroid hormone and 25-hydroxi-vitamin-D were determined in 104 patients (31 patients with chronic renal failure without renal replacement therapy, 36 patients treated with peritoneal dialysis and 37 patients treated with hemodialysis).
Introduction: There is growing evidence showing the importance of adequate vitamin D supply for preserving health.
Aim: The aim of the study was to evaluate the vitamin D supply among healthy blood donors and healthy elderly subjects in County Vas, Hungary.
Methods: Serum 25-hydroxyvitamin D, intact parathyroid hormone, calcium and albumin (Cobas, Modular, Roche), as well as serum alfa-2-globulin concentrations (Gelelfo, Interlab) were determined in 178 serum samples (68 men, 110 women, 41 were taking oral contraceptives).