Publications by authors named "Eva S Weissenberger"
B-cell acute lymphoblastic leukemia (B-ALL) occurs most commonly in children, whereas chronic myeloid leukemia is more frequent in adults. The myeloid bias of hematopoiesis in elderly individuals has been considered causative, but the age of the bone marrow microenvironment (BMM) may be contributory. Using various murine models of B-ALL in young vs old mice, we recapitulated B-ALL preponderance in children vs adults.
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- The study investigates how leukemic cells find refuge in the bone marrow and the role of specific adhesion molecules, CD44 and E-selectin, in chronic myeloid leukemia (CML).
- Researchers hypothesized that inhibiting the adhesion of CML-initiating cells to the bone marrow's E-selectin could enhance the effectiveness of imatinib treatment, and found that combining the E-selectin inhibitor GMI-1271 with imatinib improved survival in mice by reducing leukemic cell interactions with the bone marrow.
- The findings suggest that targeting specific molecular pathways, such as the modulation of adhesion molecules like CD44 and the phosphorylation of SCL/TAL1, could lead to better treatment strategies for CML in humans
Imaging of the leukemic bone marrow microenvironment, also called the leukemic bone marrow niche, is an essential method to determine and to evaluate the progression of chronic myelogenous leukemia (CML) and other leukemias in murine models. In this chapter we introduce the murine model of CML primarily used in our laboratory by describing blood and bone marrow analysis as well as the method of histological sectioning and immunohistochemistry in combination with various stainings that can help to understand the complex interaction between leukemic cells, their normal hematopoietic counterparts, and the bone marrow microenvironment. We conclude with describing how to image the bone marrow niche using in vivo microscopy.
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