Effect of calcium phosphate ceramic substrate geometry on mesenchymal stromal cell organization and osteogenic differentiation.

The composition of calcium phosphate (CaP) ceramics in combination with surface features have been shown to influence biological performance, and micro- and nano-scale topography is known to stimulate osteogenic differentiation of mesenchymal stromal cells (MSCs). In view of this, adipose tissue derived MSCs were cultured on CaP disks featuring hemispherical concavities of various sizes (440, 800 or 1800 μm diameter). It was hypothesized that (i) surface concavities would promote cell proliferation, cellular organization within the concavities, and osteogenic differentiation, as a result of a more pronounced 3D micro-environment and CaP nucleation in concavities, and (ii) MSC proliferation and osteogenic differentiation would increase with smaller concavity size due to more rapidly occurring 3D cell-cell interactions.

Influence of ceramic disk material, surface hemispheres, and SBF volume on in vitro mineralization.

Calcium phosphate ceramics are the main mineral constituents of bone and teeth and have therefore been extensively investigated for bone regenerative applications. In the current study, the effect of disk material, surface geometry, and SBF volume on mineralization capacity was investigated. Hemispherical concavities were created on the surfaces of disks made of different materials (i.

Effects of calcium phosphate composition in sputter coatings on in vitro and in vivo performance.

Calcium phosphate (CaP) ceramic coatings have been used to enhance the biocompatibility and osteoconductive properties of metallic implants. The chemical composition of these ceramic coatings is an important parameter, which can influence the final bone performance of the implant. In this study, the effect of phase composition of CaP-sputtered coatings was investigated on in vitro dissolution behavior and in vivo bone response.

Substrate geometry directs the in vitro mineralization of calcium phosphate ceramics.

Repetitive concavities on the surface of bone implants have recently been demonstrated to foster bone formation when implanted at ectopic locations in vivo. The current study aimed to evaluate the effect of surface concavities on the surface mineralization of hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) ceramics in vitro. Hemispherical concavities with different diameters were prepared at the surface of HA and β-TCP sintered disks: 1.