Antimicrobial effect of probiotic bacteriocins on biofilm in a dynamic oral flow chamber model - an study.

Aim: To determine the antimicrobial activity of the bacteriocin-producing probiotic strains K12 and M18 alone or in combination against caries-associated .

Methods: Antimicrobial activity of K12 and/or M18 against ATCC 25175 growth and biofilm formation on hydroxyapatite (HA) discs was determined in a flow chamber model by recording the colony forming units (CFU/ml) after 48 h of co-cultivation. The biofilm was analyzed by scanning electron microscopy (SEM) and by confocal laser scanning microscopy (CLSM).

Dental caries and associated factors in 7-, 12- and 15-year-old schoolchildren in the canton of Basel-Landschaft, Switzerland: Changes in caries experience from 1992 to 2021.

Background: Epidemiological surveys in schoolchildren are used to assess the current status of oral health.

Aim: To investigate the changes in caries experience among schoolchildren in the canton of Basel-Landschaft, Switzerland, over a period of three decades. Secondary objectives were to evaluate the impact of various personal and demographic factors such as age group, place of residence or dental hygiene awareness on caries prevalence as well as the history of orthodontic treatment in the year 2021.

Antibiotic Resistance among Fusobacterium, Capnocytophaga, and Leptotrichia Species of the Oral Cavity.

Purpose: Antibiotics play an important role in treating periodontal diseases. Due to the effectiveness of antibiotic therapies, their usage in dentistry has significantly increased. The aim of this study focused on the in-vitro susceptibility of different gram-negative oral bacteria species - which are associated with periodontal diseases (Fusobacterium spp.

Prevalence of and factors associated with molar-incisor hypomineralisation in schoolchildren in the canton of Basel-Landschaft, Switzerland.

Objectives: As prevalence of molar-incisor hypomineralisation varies considerably in different countries and regions, the aim of this study was to obtain representative epidemiological data for schoolchildren living in the canton of Basel-Landschaft, Switzerland.

Material And Methods: A representative population of schoolchildren of three different age groups, i.e.

Interdental biofilm reduction and composition after use of an activated and inactivated side-to-side toothbrush - a proof-of-principle clinical study.

Objectives: To evaluate interdental biofilm reduction and composition after powered toothbrushing with a side-to-side (sonic) toothbrush compared to manual toothbrushing following single brushing exercises in periodontally healthy young adults.

Materials And Methods: All participants brushed with a side-to-side toothbrush without toothpaste in four different modes: toothbrush (a) inactivated without instruction (OFF-NI), (b) activated without instruction (ON-NI), (c) inactivated with instruction (OFF-I), and (d) activated with instruction (ON-I) at consecutive visits (single brushing exercises). Before and after brushing, the Approximal Plaque Index (API) was assessed at three interdental spaces and plaque samples were taken from two interdental sites.

Awareness of vaccine-preventable diseases and self-reported immune status of Swiss dental healthcare workers.

Introduction: Dental healthcare workers are exposed to various infectious agents that may present an occupational risk. Although vaccinations rank among the most cost-effective health measures, vaccine hesitancy is present among healthcare workers.

Methods: A structured anonymous questionnaire was completed by 1111 dental healthcare workers - dentists, dental hygienists, prophylaxis assistants, dental assistants, dental technicians, and dental students.

Caries status of first-born child is a predictor for caries experience in younger siblings.

Objectives: This study analysed if children of families in need of dental interventions can be identified by using the caries status of the first-born child as a predictor for caries in younger siblings of the same family.

Material And Methods: All children aged 4 to 15 years, i.e.

Cariogenic Biofilms and Caries from Birth to Old Age.

Caries is a complex microbial disease characterized by a multifactorial etiology. The disease is driven by cariogenic microbiota that metabolize dietary carbohydrates into acids, creating prolonged periods of low pH on the biofilm surrounding the teeth, which will result in loss of calcium from the teeth leading to carious lesions. Caries remains a major public health problem globally, ranking first for the decay of permanent teeth (2.

Tolerance and Persister Formation in Oral Streptococci.

The aim of this study was to analyze the potential influence of long-term exposure in subinhibitory concentrations of chlorhexidine on the emergence of tolerant and/or persistent cells in oral streptococci. The two oral streptococcal isolates ATCC25175 and ATCC33402 were incubated, after long-term subinhibitory exposure to chlorhexidine, in liquid growth media containing high concentrations of chlorhexidine. A distinct subpopulation of more chlorhexidine-tolerant cells could be detected in streptococci that had been previously exposed to subinhibitory concentrations of chlorhexidine but not in the control strains.

Antibiotic Susceptibility Patterns of and Strains from Different Decades.

The aim of this study was to determine the antibiotic susceptibility patterns of 57 and 56 strains isolated from subgingival biofilm samples of periodontitis patients in Switzerland from 1980 to 2017. The minimal inhibitory concentrations (MIC) of the most commonly used antibiotics in periodontal therapy (amoxicillin, metronidazole, azithromycin, and doxycycline) or in severe body infections (amoxicillin/clavulanic acid, clindamycin, ertapenem, and moxifloxacin) were determined. Furthermore, all the strains were screened for beta-lactamase activity and the presence of selected resistance genes (A, F, and Q).

Surface microbial contamination in a dental department. A 10-year retrospective analysis.

Health care-associated infections may not only occur in a hospital setting, but also in dental clinics. Insufficient environmental decontamination could be one of the risk factors. In this retrospective study, we documented and analyzed the results of surface microbial contamination in a dental university-based department over an observation period of ten years.

Caries experience in 7-, 12-, and 15-year-old schoolchildren in the canton of Basel-Landschaft, Switzerland, from 1992 to 2011.

Objectives: To investigate the changes in caries experience and prevalence among schoolchildren of the canton of Basel-Landschaft, Switzerland, over the course of 20 years.

Methods: A random sample of either schoolchildren aged 7, 12, and 15 years (in 1992) or aged 12 and 15 years (in 1997) or their respective school classes (2001, 2006 and 2011) was selected so that approximately 10% of schoolchildren could be examined. The children's dmft and DMFT scores were determined according to the WHO methodology and analyzed using cluster-adjusted ordinary multiple linear regression modeling.

Development of resistance of mutans streptococci and Porphyromonas gingivalis to chlorhexidine digluconate and amine fluoride/stannous fluoride-containing mouthrinses, in vitro.

Objective: The aim if this study was to determine the minimal inhibitory concentrations of chlorhexidine digluconate and an amine fluoride/stannous fluoride-containing mouthrinse against Porphyromonas gingivalis and mutans streptococci during an experimental long-term subinhibitory exposition.

Material And Methods: Five P. gingivalis strains and four mutans streptococci were subcultivated for 20-30 passages in subinhibitory concentrations of chlorhexidine digluconate or an amine fluoride/stannous fluoride-containing mouthrinse.

Influence of time, toothpaste and saliva in the retention of Streptococcus mutans and Streptococcus sanguinis on different toothbrushes.

Objectives: The intraoral transmission of cariogenic and periodontopathogenic species seems to be facilitated by contaminated toothbrushes and other oral hygiene devices. The aim of this investigation was to analyze the in vitro retention and survival rate of Streptococcus mutans and Streptococcus sanguinis on different toothbrushes. The impacts of human saliva and antimicrobial toothpaste on these parameters were further evaluated.

Volatile compounds of Salvadora persica inhibit the growth of oral Candida species.

Objective: The antibacterial effect of Salvadora persica has been demonstrated both in vitro and in vivo. However, data on its possible antifungal effect is scarce. Therefore, the aim of the present study was to investigate the antifungal effect of solid or pulverized S.

The antimicrobial activity of alpha-bisabolol and tea tree oil against Solobacterium moorei, a Gram-positive bacterium associated with halitosis.

Objective: To investigate the antimicrobial effect of alpha-bisabolol and tea tree oil alone and in combination against the halitosis-associated Gram-positive bacillus Solobacterium moorei.

Design: The inhibitory activity of alpha-bisabolol and tea tree oil against the reference strain S. moorei CCUG39336 and four clinical S.

Additive or synergistic antimicrobial effects of amoxicillin and metronidazole on whole plaque samples: a preliminary report.

Objective: In vitro data on the susceptibility of oral bacteria to the combination of metronidazole and amoxicillin is limited. The aim of this preliminary study was to determine the susceptibility of whole subgingival plaque samples to amoxicillin and metronidazole and to their combination.

Methods: Prior to any treatment procedures subgingival plaque samples from patients with severe generalized periodontitis were taken.

Antimicrobial efficacy of 3 oral antiseptics containing octenidine, polyhexamethylene biguanide, or Citroxx: can chlorhexidine be replaced?

Background: Use of oral antiseptics decreases the bacterial load in the oral cavity.

Objective: To compare the antimicrobial activity of 3 novel oral antiseptics with that of chlorhexidine, which is considered the "gold standard" of oral hygiene.

Design: Comparative in vitro study.

Antimicrobial susceptibility of periodontopathogenic bacteria.

Objectives: The aim of this study was to evaluate the resistance profiles of Aggregatibacter (Actinobacillus) actinomycetemcomitans, Porphyromonas gingivalis and Prevotella intermedia/Prevotella nigrescens and to detect possible changes in antibiotic resistance over the time period of 1991-2005.

Methods: A. actinomycetemcomitans (125 strains), P.

Adhesion of Streptococcus sanguinis to dental implant and restorative materials in vitro.

Bacterial adhesion to tooth surfaces or dental materials starts immediately upon exposure to the oral environment. The aim of this study, therefore, was to compare the adhesion of Streptococcus sanguinis to saliva-coated human enamel and dental materials - during a one-hour period - using an in vitro flow chamber system which mimicked the oral cavity. After fluorescent staining, the number of adhered cells and their vitality were recorded.

Clinical effectiveness of two different toothbrushes in the elderly.

Proper tooth brushing is the simplest way to maintain oral health. Still, it can cause considerable manipulative difficulties among parts of the population, like the elderly. The aim of this study was to evaluate a tool that could make tooth-brushing more effective and easier for this age group.

Complete genomic nucleotide sequence of the temperate bacteriophage Aa Phi 23 of Actinobacillus actinomycetemcomitans.

The entire double-stranded DNA genome of the Actinobacillus actinomycetemcomitans bacteriophage Aa Phi 23 was sequenced. Linear DNA contained in the phage particles is circularly permuted and terminally redundant. Therefore, the physical map of the phage genome is circular.

[Ribotyping Fusobacterium nucleatum isolates from healthy and diseased periodontium].

Fusobacterium nucleatum is one of the most frequently cultured bacteria from subgingival plaque. It can be isolated from active periodontal lesions as well as from healthy sites. Currently, F.