Batch Production of High-Quality Graphene Grids for Cryo-EM: Cryo-EM Structure of Soluble Methane Monooxygenase Hydroxylase.

Cryogenic electron microscopy (cryo-EM) has become a widely used tool for determining the protein structure. Despite recent technical advances, sample preparation remains a major bottleneck for several reasons, including protein denaturation at the air-water interface, the presence of preferred orientations, nonuniform ice layers, etc. Graphene, a two-dimensional allotrope of carbon consisting of a single atomic layer, has recently gained attention as a near-ideal support film for cryo-EM that can overcome these challenges because of its superior properties, including mechanical strength and electrical conductivity.

Development of an atmospheric plasma jet device for versatile treatment of electron microscope sample grids.

Atmospheric-pressure plasmas have been widely applied for surface modification and biomedical treatment because of their ability to generate highly reactive radicals and charged particles. In negative-stain electron microscopy (Neg-EM) and cryogenic electron microscopy (cryo-EM), plasmas have been used to generate hydrophilic surfaces and eliminate surface contaminants to embed specimens onto grids. In addition, plasma treatment is a prerequisite for negative-stain and Quantifoil grids, whose surfaces are coated with hydrophobic amorphous carbon.

A4 Paper Chemistry: Synthesis of a Versatile and Chemically Modifiable Cellulose Membrane.

Multifunctional cellulose membranes were developed from A4-sized printing paper via chemical modification. A4 paper is a widely used and easily accessible product with high cellulose content. Inspired by cellulose chemistry, we report a simple modification of the A4 paper, converting it from a common office supply to a user-modifiable functionalized cellulose membrane for practical applications.

Layer-by-Layer Assembly: Recent Progress from Layered Assemblies to Layered Nanoarchitectonics.

As an emerging concept for the development of new materials with nanoscale features, nanoarchitectonics has received significant recent attention. Among the various approaches that have been developed in this area, the fixed-direction construction of functional materials, such as layered fabrication, offers a helpful starting point to demonstrate the huge potential of nanoarchitectonics. In particular, the combination of nanoarchitectonics with layer-by-layer (LbL) assembly and a large degree of freedom in component availability and technical applicability would offer significant benefits to the fabrication of functional materials.

Reduction-Triggered Self-Cross-Linked Hyperbranched Polyglycerol Nanogels for Intracellular Delivery of Drugs and Proteins.

Owing to the unique advantages of combining the characteristics of hydrogels and nanoparticles, nanogels are actively investigated as a promising platform for advanced biomedical applications. In this work, a self-cross-linked hyperbranched polyglycerol nanogel is synthesized using the thiol-disulfide exchange reaction based on a novel disulfide-containing polymer. A series of structural analyses confirm the tunable size and cross-linking density depending on the type of polymer (homo- or copolymer) and the amount of reducing agent, dithiothreitol, used in the preparation of the nanogels.

Bioinspired Catecholic Primers for Rigid and Ductile Dental Resin Composites.

In the construction of dental restorative polymer composite materials, surface priming on mineral fillers is essential to improve the mechanical performance of the composites. Here we present bioinspired catechol-functionalized primers for a tougher dental resin composite containing glass fillers. The catecholic primers with different polymerizable end groups were designed and then coated on glass surfaces using a simple drop-casting or dip-coating process.

Multidimensional Thin Film Hybrid Electrodes with MoS Multilayer for Electrocatalytic Hydrogen Evolution Reaction.

Hybrid electrodes are widely used in various energy storage and conversion devices. However, conventional fabrication methods like simple mixing allow only limited control over the internal electrode structure, and it is often difficult to elucidate the structure-property relationship among the electrode components. Taking advantage of the versatile layer-by-layer (LbL) assembly method, herein we report the preparation of electrocatalytic thin film electrodes for hydrogen evolution reaction (HER), highlighting the importance of nanoscale composition in multidimensional hybrid electrodes.