Role of high-temperature requirement serine protease A 2 in rheumatoid inflammation.

Background: High-temperature requirement serine protease A 2 (HtrA2) is known to be involved in growth, unfolded protein response to stress, apoptosis, and autophagy. However, whether HtrA2 controls inflammation and immune response remains elusive.

Methods: Expression of HtrA2 in the synovial tissue of patients was examined using immunohistochemistry and immunofluorescence staining.

Identification of MYH9 as a key regulator for synoviocyte migration and invasion through secretome profiling.

Objectives: 'Invasive pannus' is a pathological hallmark of rheumatoid arthritis (RA). This study aimed to investigate secretome profile of synovial fibroblasts of patients with RA (RA-FLSs), a major cell type comprising the invasive pannus.

Methods: Secreted proteins from RA-FLSs were first identified using liquid chromatography-tandem mass spectrometry analysis.

Regulation of tumor growth by leukocyte-specific protein 1 in T cells.

Background: Clinical efficacy of T cell-based cancer immunotherapy is limited by the lack of T cell infiltration in the tumor mass, especially in solid tumors. Our group demonstrated previously that leukocyte-specific protein 1 (LSP1), an intracellular signal regulator, negatively regulates T cell infiltration in inflamed tissues.

Methods: To determine the immuno-regulatory effects of LSP1 in T cells on tumor progression, we investigated the growth of B16 melanoma in knockout (KO) mice and T cell-specific transgenic (Tg) mice.