Ultrasound-guided intratumoral indigo carmine injection for intraoperative, surgeon-performed tumor localization.

Background: The purpose of this study was to evaluate the feasibility of ultrasound-guided intratumoral indigo carmine injections for intraoperative localization of small head and neck tumors and to introduce our surgeon-performed technique.

Methods: In total, 23 patients who had tumors ≤1.5 cm that were not palpable by 2 head and neck surgeons were enrolled in this prospective study.

Redox-responsive in vitro modulation of the signalling state of the isolated PrrB sensor kinase of Rhodobacter sphaeroides NCIB 8253.

Prr is a global regulatory system that controls a large and diverse range of genes in Rhodobacter sphaeroides in response to changing conditions of environmental redox potential. PrrB is the membrane-bound sensor kinase and previously we showed that the purified, detergent-solubilised intact membrane protein is functional in autophosphorylation, phosphotransfer and phosphatase activities. Here we confirm that it also senses and responds directly to its environmental signal, redox potential; strong autophosphorylation of PrrB occurred in response to dithiothreitol (DTT)-induced reducing conditions (and levels increased in response to a wide 0.