Publications by authors named "Eun Young Kuak"
Article Synopsis
- - The study compared two PCR tests (BD GeneOhm and Seegene ACE) for detecting the tcdB gene from stool samples, finding a high agreement rate of 96.3% between the two methods.
- - BD GeneOhm demonstrated sensitivities of 95.7% and specificities of 96.5%, while Seegene ACE had sensitivities of 90.0% and specificities of 97.1%.
- - Positive predictive values (PPVs) were 91.8% for BD and 92.6% for Seegene, while negative predictive values (NPVs) were 98.2% and 96.0%, respectively.
Article Synopsis
- This study evaluates the new VIDAS C. difficile Toxin A & B assay (CDAB) against the existing VIDAS C. difficile Toxin A II assay (CDA) for diagnosing C. difficile infections in areas where TcdA is not present but TcdB is prevalent.
- Out of 555 fecal samples, 150 were positive for C. difficile, showing an 81.8% concordance between the two assays, with the CDAB demonstrating significantly higher sensitivity (65.3%) compared to CDA (29.8%).
- The CDAB assay was more effective at detecting both toxin A and B strains, identifying a greater percentage of the tcdA(+)tcdB(+) and
Enzyme immunoassays (EIA) to detect glutamate dehydrogenase or toxins A (TcdA) and B (TcdB), a cytotoxicity assay, and bacteriologic culture have disadvantages when applied individually to diagnosis of Clostridium difficile infections. Stool specimens (n = 1,596) were subjected to toxin detection via an enzyme-linked fluorescent immunoassay (ELFA; Vidas CDAB assay) and bacteriologic culture for toxigenic C. difficile in a three-step algorithm with additional toxigenic culture.
View Article and Find Full Text PDFThe prevalence of toxigenic Clostridium difficile in Korea has been reported to be approximately 60-80%. Although the prevalence of the tcdA(-)tcdB(+) C. difficile strain was less then 5% prior to the year 2000, it has become an emerging nosocomial pathogen in Korea.
View Article and Find Full Text PDFBackground: We intended to evaluate the diagnostic usefulness of a multiplex reverse transcriptase- PCR (mRT-PCR) assay kit under dual priming oligonucleotide system (DPO) for the childhood acute respiratory tract infections.
Methods: Two hundred nasopharyngeal aspirates were taken from children < or =5 yr old admitted due to acute respiratory infections in 2004. Direct fluorescent antibody (FA) assays were performed with fresh specimens; then, mRT-PCRs for the detection of 12 respiratory viruses (Seeplex RV detection kit, SeeGene, Seoul, Korea) were tested with frozen specimens.
[Characterization of a Toxin A-Negative, Toxin B-Positive Variant Strain of Clostridium difficile.].
Korean J Lab Med
February 2006
Background: Clostridium difficile is one of the most important pathogens responsible for nosocomial diarrhea. Recently, we have frequently experienced culture positive, toxin A enzyme immunoassay negative strains. Therefore, we evaluated the strains with several PCR primer sets to characterize them.
View Article and Find Full Text PDFSixty percent to 80% of Clostridium difficile isolates in Korea have been reported to be toxigenic. However, over 1 year, we encountered a high number of tcdA-tcB+ strains associated with pseudomembranous colitis (PMC). C.
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