Publications by authors named "Eun Sol Chang"
Article Synopsis
- Mitochondria play a crucial role in producing ATP, and measuring their synthesis rate can reveal insights into cell energy levels, but traditional methods have limitations in assessing real-time changes.
- The MitoRAISE assay was developed to detect these real-time ATP level changes by analyzing the response of mitochondrial ATP synthesis to different substrates, using various cell conditions to validate its effectiveness.
- Results indicated that mitochondrial function (ATP levels and responses) was impaired in breast cancer patients compared to healthy individuals, suggesting that MitoRAISE could serve as a valuable tool for monitoring mitochondrial health in various diseases.
Background: Endocrine therapy resistance in hormone receptor-positive/HER2-negative (HR+/HER2-) breast cancer (BC) is a significant clinical challenge that poses several unmet needs in the management of the disease. This study aimed to investigate the prognostic value of c-MET-positive circulating tumor cells (cMET+ CTCs), ESR1/PIK3CA mutations, and cell-free DNA (cfDNA) concentrations in patients with hormone receptor-positive (HR+) metastatic breast cancer (mBC).
Methods: Ninety-seven patients with HR+ mBC were prospectively enrolled during standard treatment at Samsung Medical Center.
Article Synopsis
- * Researchers explored the use of small interfering RNAs (siRNAs) targeting the BRD4-NUTM1 fusion (B4N), the most prevalent type in NC, to assess their potential as a treatment option and evaluated patient blood samples for treatment response.
- * The study found that B4N-specific siRNA effectively inhibited the fusion transcript and protein, reducing cell growth and tumor size, while levels of the fusion transcript in patient samples correlated with treatment responsiveness, indicating a pathway for precision medicine in managing NC.
Detection of oncogenic fusion genes in cancers, particularly in the diagnosis of uncertain tumors, is crucial for determining effective therapeutic strategies. Although novel fusion genes have been discovered through sequencing, verifying their oncogenic potential remain difficult. Therefore, we evaluated the utility of targeted RNA sequencing in 165 tumor samples by identifying known and unknown fusions.
View Article and Find Full Text PDFPurpose: The purpose of this study was to investigate the concordance rate of PIK3CA mutations between primary and matched distant metastatic sites in patients with breast cancer and to verify whether there are differences in the frequency of PIK3CA hotspot mutations depending on the metastatic sites involved.
Materials And Methods: Archived formalin-fixed paraffin-embedded (FFPE) specimens of primary breast and matched distant metastatic tumors were retrospectively obtained for 49 patients. Additionally, 40 archived FFPE specimens were independently collected from different breast cancer metastatic sites, which were limited to three common sites: the liver, brain, and lung.
Purpose: To find biomarkers for disease, there have been constant attempts to investigate the genes that differ from those in the disease groups. However, the values that lie outside the overall pattern of a distribution, the outliers, are frequently excluded in traditional analytical methods as they are considered to be 'some sort of problem.' Such outliers may have a biologic role in the disease group.
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