Publications by authors named "Eun Ji Ha"
Article Synopsis
- Drug solubility limits how well poorly water-soluble medicines can be administered via intravenous injection, affecting their bioavailability estimates.
- Researchers tested a new method using stable isotope tracers to more accurately assess the bioavailability of poorly water-soluble drugs, using HGR4113 and its deuterated version HGR4113-d7 as examples.
- The study developed a bioanalytical technique using LC-MS/MS to measure plasma levels of both compounds in rats, revealing that HGR4113's bioavailability was around 53.3% to 67.8% at different oral doses, and the new approach significantly reduced errors in bioavailability measurement compared to traditional methods.
Interests in the development and exploration of industrial applications of medicinal mushrooms as postbiotics have lately increased. We recently reported the potential use of Phellinus linteus mycelial-containing whole culture extract (PLME) prepared by submerged cultivation as a postbiotic that promotes immune system activation. Here, we aimed to isolate and structurally elucidate the active ingredients in PLME by activity-guided fractionation.
View Article and Find Full Text PDFMedicinal mushrooms are an important natural resource promoting health benefits. Herein, mycelia were prepared under submerged cultivation, the mycelium-containing culture broth was extracted as a whole to obtain the postbiotic materials (PLME), and its effect on the immune system was evaluated in normal C3H/HeN mice. Oral administration of PLME for 4 weeks was well tolerated and safe.
View Article and Find Full Text PDFBACKGROUND The flexibility of the long flexometallic tube makes insertion of the flexible reinforced laryngeal mask airway (f-LMA) difficult. We compared the usefulness of rigid lightwand-guided f-LMA insertion with standard digital manipulation. MATERIAL AND METHODS Fifty-four patients (aged 19-70 years) were randomly divided into a control group (digital manipulation technique) or the lightwand group (lightwand-guided insertion technique).
View Article and Find Full Text PDFA flavonoid antioxidant quercetin promotes dose-dependent activation of the ATM-CHK-p53 pathway, downregulation of antiapoptotic survivin, and upregulation of proapoptotic NOXA in human T cell acute lymphoblastic leukemia Jurkat clones (J/Neo and J/BCL-XL). However, the downregulation of antiapoptotic BAG3 and MCL-1 occurred in J/Neo cells but not in J/BCL-XL cells overexpressing BCL-XL. Additionally, several BCL-XL-sensitive intrinsic mitochondrial apoptotic events including apoptotic sub-G cell accumulation, TUNEL-positive DNA fragmentation, BAK activation, mitochondrial membrane potential (m) loss, caspase-9/caspase-8/caspase-3 activation, and PARP cleavage were induced only in J/Neo cells.
View Article and Find Full Text PDFArticle Synopsis
- Acacetin, a compound found in barnyard millet, triggers cytotoxic and apoptotic responses in Jurkat T cells, including Bak activation and loss of mitochondrial membrane potential, without causing necrosis.
- Apoptosis is reduced in cells overexpressing Bcl-xL, while autophagy markers are affected differently; only non-Bcl-xL cells show significant autophagic responses like Beclin-1 phosphorylation.
- Inhibition of autophagy enhances apoptosis features, indicating that acacetin promotes both apoptosis and protective autophagy in T cells, with potential implications for cancer treatment strategies.