Publications by authors named "Eun Ah Ko"
High-fructose corn syrup (HFCS) is widely used as sweetener, and its overconsumption is become a major health problem. In the present study, we used adult female rats and applied a 28 days HFCS feeding model to monitor the estrous cycle and changes in tissue weights and histology. Adult female rats were divided into three groups.
View Article and Find Full Text PDFArticle Synopsis
- 114 Acinetobacter sp. isolates were collected from emergency departments in two Korean hospitals, with 92.1% belonging to the Acinetobacter baumannii complex.
- Imipenem resistance was observed in 34.2% of the isolates, with additional cases of colistin resistance, highlighting significant antimicrobial resistance issues.
- Species distribution varied between hospitals, with distinct genetic profiles found in imipenem-resistant isolates, emphasizing the need for ongoing monitoring of these bacteria in healthcare settings.
The open reading frame of dsrE563, a dextransucrase gene obtained from a constitutive mutant (CB4-BF563) of Leuconostoc mesenteroides B-1299, consists of 8,511 bp encoding 2,836 amino acid residues. DsrE563 contains two catalytic domains (CD1 and CD2). Two truncated derivative mutants DsrE563ΔCD2ΔGBD (DsrE563-1) and DsrE563ΔCD2ΔVR (DsrE563-2) of DsrE563 were constructed and expressed using the pRSETC vector in Escherichia coli.
View Article and Find Full Text PDFArticle Synopsis
- A study was conducted on Acinetobacter species isolates from various environments, including soil, focusing on species identification and antimicrobial resistance.
- Fourteen species of Acinetobacter were identified among 29 isolates, with several being resistant to important antibiotics like imipenem and colistin.
- The findings suggest that genetically distinct Acinetobacter species in natural environments could pose a public health threat, highlighting the concern of antimicrobial resistance.
An artificial fusion protein of Arthrobacter oxydans dextranase and Klebsiella pneumoniae alpha-amylase was constructed and expressed in Escherichia coli. Most of the expressed protein existed as an insoluble fraction, which was solubilized with urea. The purified fusion enzyme electrophoretically migrated as a single protein band; M = 137 kDa, and exhibited activities of both dextranase (10.
View Article and Find Full Text PDFA novel dextransucrase gene, DSRN, was obtained by ultrasoft X-ray treatment of the DSRB742 gene. The DSRN gene was further mutated via site-directed mutagenesis producing four mutants: DSRN1 (F196S), DSRN2 (Y346N), DSRN3 (K395T) and DSRN4 (P980T). Dextransucrases derived from DSRB742 and its mutants were expressed in E.
View Article and Find Full Text PDF