Complete genome sequence of strain TDY-1, an odor-reducing bacterium isolated from livestock wastewater.

We report the complete genome sequence of TDY-1 an odor-reducing strain isolated from livestock wastewater on a pig farm. Its genome consists of a 2.97-Mb chromosome.

Complete genome sequence of strain JD2-26, a potential pollutant-reducing bacterium isolated from a municipal solid waste landfill facility.

We report here the complete genome sequence of strain JD2-26 isolated from a municipal solid waste landfill facility. The genome consists of a 6.32-Mbp chromosome and a plasmid having a total of 6,100 genes, including 5,914 coding sequences.

Complete genome sequence of a methanotrophic bacterium, strain 22M6SE5-12 isolated from a freshwater sediment environment.

We assembled the complete genome of strain 22M6SE5-12 isolated from a freshwater sediment in South Korea. The genome consists of a 4.36-Mbp chromosome and two plasmids and has 3,961 coding sequences, 12 rRNA genes, and 55 tRNA genes.

Enhanced astaxanthin production in Haematococcus lacustris by electrochemical stimulation of cyst germination.

This study investigated the technical feasibility of using electrogermination to activate dormant cysts as an inoculum for subsequent 14-d photosynthetic astaxanthin production in Haematococcus lacustris. Electrotreatment affected the cell viability, surface charge, and morphology of H. lacustris cysts.

sp. nov., isolated from sediment in a freshwater pond.

A Gram-stain-negative, aerobic, oxidase-positive, weakly catalase-positive, motile by means of a single polar flagellum, rod-shaped bacterium designated as strain S2-9 was isolated from sediment sampled in Wiyang pond, Republic of Korea. Growth of this strain was observed at 10-40 °C (optimum, 35 °C) and pH 5.5-9.

Community recovery of benthic macroinvertebrates in a stream influenced by mining activity: Importance of microhabitat monitoring.

The decrease in freshwater biodiversity owing to anthropogenic disturbances such as mining activity is a global challenge; hence, there is an urgent need for systematic approaches to continuously monitor such disturbances and/or the recovery of biodiversity in freshwater habitats. The Hwangjicheon Stream is the source of South Korea's longest river and has been subjected to runoff from coal mining. We investigated changes in the diversity of the benthic macroinvertebrate community in various microhabitats, including riffle, run, and pool, to monitor the recovery of biodiversity in the stream following the improvement of a mining water treatment plant in 2019.

Chlorophyll Synthesis in by Chlorophyll Synthase of .

The production of phytylated chlorophyll (Chl ) in , which uses phytylated bacteriochlorophyll (BChl ), is the first step in expanding the light absorption spectra. Unlike the chlorophyll synthase (ChlG) of the sp. PCC6803, ChlGs of angiosperms, including , , , and , showed bacteriochlorophyll synthase activity and resistance to inhibition by bacteriochlorophyllide (BChlide ), geranylgeranylated BChl (BChl ), and BChl , collectively called bacteriochlorins.

The Photoactive Photosynthetic Reaction Center of a Rhodobacter sphaeroides Mutant Lacking 3-Vinyl (Bacterio)Chlorophyllide Hydratase Contains 3-Vinyl Bacteriochlorophyll .

Rhodobacter sphaeroides mutant BF-lacking 3-vinyl (bacterio)chlorophyllide hydratase (BchF)-accumulates chlorophyllide (Chlide ) and 3-vinyl bacteriochlorophyllide (3V-Bchlide ). BF synthesizes 3-vinyl bacteriochlorophyll (3V-Bchl ) through prenylation of 3V-Bchlide and assembles a novel reaction center (V-RC) using 3V-Bchl and Mg-free 3-vinyl bacteriopheophytin (3V-Bpheo ) at a molar ratio of 2:1. We aimed to verify whether a -deleted R.

Photoautotrophic Growth Rate Enhancement of sp. PCC6803 by Heterologous Production of 2-Oxoglutarate:Ferredoxin Oxidoreductase from .

2-Oxoglutarate:ferredoxin oxidoreductase from Chlorobaculum tepidum (CtOGOR) is a carbon-fixing enzyme in the reductive TCA cycle that reversibly carboxylates succinyl-CoA to yield 2-oxoglutarate. CtOGOR is a heterotetramer of two large (α = 68 kDa) and two small (β = 38 kDa) subunits. The αβ protomer harbors one thiamine pyrophosphate and two 4Fe-4S clusters.

and phototoxicity on gastric mucosa induced by methylene blue.

Background: Methylene blue (MB) is used endoscopically to demarcate tumors and as a photosensitizer in photodynamic therapy (PDT). However, there are few in vivo studies about its toxicity in healthy stomach tissue. We performed sequential in vitro and in vivo analyses of MB-induced phototoxicity.

Sustainability of in vitro light-dependent NADPH generation by the thylakoid membrane of Synechocystis sp. PCC6803.

Background: NADPH is used as a reductant in various biosynthetic reactions. Cell-free bio-systems have gained considerable attention owing to their high energy utilization and time efficiency. Efforts have been made to continuously supply reducing power to the reaction mixture in a cyclical manner.

Multi-scale causality analysis between COVID-19 cases and mobility level using ensemble empirical mode decomposition and causal decomposition.

The global spread of the coronavirus disease 2019 (COVID-19) pandemic has affected the world in many ways. Due to the communicable nature of the disease, it is difficult to investigate the causal reason for the epidemic's spread sufficiently. This study comprehensively investigates the causal relationship between the spread of COVID-19 and mobility level on a multi time-scale and its influencing factors, by using ensemble empirical mode decomposition (EEMD) and the causal decomposition approach.

Evaluation of the Impacts of Abandoned Mining Areas: A Case Study with Benthic Macroinvertebrate Assemblages.

Mining activities are among the most long-lasting anthropogenic pressures on streams and rivers. Therefore, detecting different benthic macroinvertebrate assemblages in the areas recovered from mining activities is essential to establish conservation and management plans for improving the freshwater biodiversity in streams located near mining areas. We compared the stability of benthic macroinvertebrate communities between streams affected by mining activities (Hwangjicheon: NHJ and Cheolamcheon: NCA) and the least disturbed stream (Songjeonricheon: NSJ) using network analysis, self-organizing map, and indicator species analysis.

Application of naïve Bayesian approach in detecting reproducible fatal collision locations on freeway.

Detecting high-collision-concentration locations based solely on collision frequency may produce different results compared to those considering the severities of the collisions. In particular, it can lead government agencies focusing sites with a high collision frequency while neglecting those with a lower collision frequency but a higher percentage of injury and fatal collisions. This study developed systematic ways of detecting reproducible fatal collision locations (R) using the naïve Bayes approach and a continuous risk profile (CRP) that estimates the true collision risk by filtering out random noise in the data.

Anti-Inflammatory Effects of a Polyphenol, Catechin-7,4'--Digallate, from by Regulating NF-κB Signaling Pathway in Mouse Macrophages.

Inflammation is a defense mechanism that protects the body from infections. However, chronic inflammation causes damage to body tissues. Thus, controlling inflammation and investigating anti-inflammatory mechanisms are keys to preventing and treating inflammatory diseases, such as sepsis and rheumatoid arthritis.

Spatiotemporal filtering method for detecting kinematic waves in a connected environment.

Backward-moving kinematic waves (KWs) (e.g., stop-and-go traffic conditions and a shock wave) cause unsafe driving conditions, decreases in the capacities of freeways, and increased travel time.

Differences in Mechanisms of Steroid Therapy and Olfactory Training for Olfactory Loss in Mice.

Objective: Steroid therapy and olfactory training are common treatments for olfactory loss. Systemic steroid treatment is the most effective approach for treating sinonasal olfactory loss. Olfactory training is typically effective for treating sensorineural olfactory loss.

Bactericidal Effects of Photodynamic Therapy Combined with Four Tetracyclines against KCTC5009 in Planktonic Cultures.

Surface disinfection in health-care facilities is critical to prevent dissemination of (). Tetracyclines (TCs) are broad-spectrum antibiotics that are associated with a low risk of development of infection (CDI) and are used as photosensitizers (PS) in photodynamic therapy (PDT). We evaluated whether TCs may be useful environmental cleansing agents.

Preparation of Radiation Cross-Linked Poly(Acrylic Acid) Hydrogel Containing Metronidazole with Enhanced Antibacterial Activity.

Metronidazole (MD) is known as a periodontitis medicine and has been widely used in antibiotics for resistance to anaerobic bacteria, periodontal disease, and other threats. To treat diseases, drug delivery carriers are needed with a high bioadhesive property and enhanced drug penetration. Poly (acrylic acid) (PAA) hydrogel films have a good bioadhesive property and are able to localize the absorption site and increase the drug residence time.

Comparison of high and low molecular weight chitosan as in-vitro boosting agent for photodynamic therapy against Helicobacter pylori using methylene blue and endoscopic light.

Background: We reported in a previous study that photodynamic therapy (PDT) of Helicobacter pylori(H. pylori) could potentiate bactericidal effect by adding chitosan. As a next step, we compared the bactericidal effects of low molecular weight (LMW) combined with Photodynamic Therapy to high molecular weight (HMW) chitosan.

The neuroplastic effect of olfactory training to the recovery of olfactory system in mouse model.

Background: Several studies have reported the benefits of olfactory training (OT) in the olfactory nervous system of mouse models. Therefore, in this study we performed next-generation sequencing to evaluate the effects of OT on mRNA sequencing in the olfactory area.

Methods: Mice in each group were administered 300 mg of 3-methylindole per kilogram of mouse weight.

Building a Thermostable Metabolon for Facilitating Coenzyme Transport and In Vitro Hydrogen Production at Elevated Temperature.

To facilitate coenzyme transport and in vitro enzymatic hydrogen production, a multi-enzyme metabolon comprising a miniscaffoldin containing three cohesins, a dockerin-containing mutant dehydrogenase, a dockerin-containing diaphorase, and a Histidine-tagged (His-tagged) NiFe hydrogenase was constructed. As the NiFe hydrogenase has very complicated structure and cannot be fused directly with a dockerin, a bifunctional peptide was designed. The bifunctional peptide, in which one terminus contains a modified dockerin binding the cohesin of the miniscaffoldin and the other, after chemical modification, binds the His-tag of NiFe hydrogenase, enabled His-tagged proteins to be integrated into the cohesin-dockerin-based metabolon.

Advanced water splitting for green hydrogen gas production through complete oxidation of starch by in vitro metabolic engineering.

Starch is a natural energy storage compound and is hypothesized to be a high-energy density chemical compound or solar fuel. In contrast to industrial hydrolysis of starch to glucose, an alternative ATP-free phosphorylation of starch was designed to generate cost-effective glucose 6-phosphate by using five thermophilic enzymes (i.e.