Chemical Relationship among Genetically Authenticated Medicinal Species of Genus Angelica.

The genus comprises various species utilized for diverse medicinal purposes, with differences attributed to the varying levels or types of inherent chemical components in each species. This study employed DNA barcode analysis and HPLC analysis to genetically authenticate and chemically classify eight medicinal species ( = 106) as well as two non-medicinal species ( = 14) that have been misused. Nucleotide sequence analysis of the nuclear internal transcribed spacer (ITS) region revealed differences ranging from 11 to 117 bp, while showed variances of 3 to 95 bp, respectively.

Chemotaxonomic Classification of and Its Chemical Correlation with , , and by Liquid Chromatography Combined with Chemometrics.

The roots of (Apiaceae) have been used as an alternative to the roots of (Apiaceae) to treat common cold-related symptoms in Korea. However, a variety of species, including the roots of or (=), have been used to treat phlegm-heat-induced symptoms in China. Hence, as there are differences in the medicinal application of roots between Korea and China, chemotaxonomic classification of was evaluated.

Quantitative Comparison of the Marker Compounds in Different Medicinal Parts of L. Using High-Performance Liquid Chromatography-Diode Array Detector with Chemometric Analysis.

It is thought that the therapeutic efficacy of L. is determined by its biological compounds. We investigated the chemical differences in the medicinal parts of by analyzing a total of 57 samples (15 root barks, 11 twigs, 12 fruits, and 19 leaves).

Chemotaxonomic Monitoring of Genetically Authenticated Amomi Fructus Using High-Performance Liquid Chromatography-Diode Array Detector with Chemometric Analysis.

Amomi Fructus is widely used to treat digestive disorders, and , var. , and are permitted medicinally in national pharmacopeias. However, there are a variety of adulterants present in herbal markets owing to their morphological similarities to the genuine species.

DNA Markers to Discriminate L. 'Cheungsam' with Low Tetrahydrocannabinol (THC) Content from Other South Korea Cultivars Based on the Nucleotide Sequences of and Putative Genes.

L. has been utilized for a long time as a traditional herbal medicine in Korea. Dry fruits, achenes, each containing a single seed of , are currently prescribed as (Cannabis Semen), a laxative.

Identification and Monitoring of Amomi Fructus and its Adulterants Based on DNA Barcoding Analysis and Designed DNA Markers.

Amomi Fructus is one of the traditional medicines derived from the ripe fruits of the Zingiberaceae family of plants, which include , var. , and . Owing to their highly similar morphological traits, several kinds of adulterants of Amomi Fructus have been reported.

Quantitative Interrelation between Atractylenolide I, II, and III in Koidzumi Rhizomes, and Evaluation of Their Oxidative Transformation Using a Biomimetic Kinetic Model.

Analytical methods based on ultraperformance liquid chromatography/ion-trap mass spectrometry (UPLC/ion-trap MS) were developed for quantification of atractylenolide I, II, and III in the methanol extract of rhizomes with a C column in an acidified water/acetonitrile gradient eluent in an LC system, and ion-trap MS coupled with electrospray ionization was employed under positive-ion mode. The three atractylenolides were quantified in all samples, and the content of atractylenolide I, II, and III showed a significant correlation to each other. Such high correlation was explained by the mechanistic insights into the biosynthetic pathway of atractylenoide III and I from atractylenoide II by using the biomimetic cytochrome P450 model, [Fe(tmp)](CFSO) (tmp = -tetramesitylporphyrin).

Chemical Differentiation of Genetically Identified , , and Rhizomes Using High-Performance Liquid Chromatography with Chemometric Analysis.

The rhizome of , which is a herbal medicine used for gastrointestinal therapeutics, has been categorized with rhizome or rhizome based on different therapeutic criteria in Korea, China, and Japan. In the present study, 61 , , and rhizomes were collected from Korea and China and were genetically identified by internal transcribed spacer sequencing analysis. Chromatographic profiles were obtained from high-performance liquid chromatography analysis of the methanol and hot-water extracts of rhizomes and chemical differentiation of the rhizomes was carried out using chemometric statistical analyses such as principal component analysis, hierarchical clustering analysis, and Pearson's correlation coefficient analysis.

Application of Partial Internal Transcribed Spacer Sequences for the Discrimination of Artemisia capillaris from Other Artemisia Species.

Several Artemisia species are used as herbal medicines including the dried aerial parts of Artemisia capillaris, which are used as Artemisiae Capillaris Herba (known as "Injinho" in Korean medicinal terminology and "Yin Chen Hao" in Chinese). In this study, we developed tools for distinguishing between A. capillaris and 11 other Artemisia species that grow and/or are cultured in China, Japan, and Korea.

Evaluation of Medicinal Categorization of Atractylodes japonica Koidz. by Using Internal Transcribed Spacer Sequencing Analysis and HPLC Fingerprinting Combined with Statistical Tools.

Atractylodes rhizomes have been used as the herbal medicine "Changchul" or "Baekchul," according to their clinical purpose, in Korea, China, and Japan. Among the Atractylodes species, the medicinal use of Atractylodes japonica has been controversial, as it is categorized as both Changchul and Baekchul in those countries, and, moreover, parts of the rhizome have been differently used, depending on age of the plant, in Korea. Chromatographic fingerprinting by using HPLC combined with chemometric analyses and internal transcribed spacer (ITS) sequencing analysis were conducted to classify and identify 34 crude drugs derived from Atractylodes rhizomes.

Multiplex PCR method to discriminate Artemisia iwayomogi from other Artemisia plants.

Some plants in the genus Artemisia have been used for medicinal purposes. Among them, Artemisia iwayomogi, commonly referred to as "Haninjin," is one of the major medicinal materials used in traditional Korean medicine. By contrast, Artemisia capillaris and both Artemisia argyi and Artemisia princeps, referred to as "Injinho" and "Aeyup," respectively, are used to treat diseases different from those for which "Haninjin" is prescribed.

Application of the multiplex PCR method for discrimination of Artemisia iwayomogi from other Artemisia herbs.

Some plants classified in the genus Artemisia are used for medicinal purposes. In particular, A. iwayomogi, which is referred to as 'Haninjin,' is used as an important medicinal material in traditional Korean medicine.

Development of SCAR marker for discrimination of Artemisia princeps and A. argyi from other Artemisia herbs.

Some Artemisia herbs are used for medicinal purposes. In particular, A. princeps and A.